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Análise estrutural das proteínas da seda da teia da aranha Nephila clavipes por uma abordagem proteômica

Pinto, José Roberto Aparecido dos Santos
Fonte: Universidade Estadual Paulista (UNESP) Publicador: Universidade Estadual Paulista (UNESP)
Tipo: Tese de Doutorado Formato: 287 f. : il., tabs.
Português
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Processo FAPESP: 10/19051-6; Pós-graduação em Ciências Biológicas (Biologia Celular e Molecular) - IBRC; The orb-web spiders became efficient producers of different types of silks. These silks are characterized by diversity in their chemical composition, structure and function, ranging from the construction of the orb-web to the cocoon. Spider web silk proteins (spidroins) have interesting relationships between their 3-D structures and mechanical properties of these protein fibers, which are secreted by specialized abdominal glands. The major ampullate silk fibers, produced by major ampullate gland, are one of the most important types of fibers spun produced by the orb-web spiders of genus Nephila, and are nanostructured composite materials predominantly composed of two structural proteins, designated spidroin-1 and -2. While the fibers produced by the flagelliform gland consist of only a single protein, the flagelliform silk protein. Despite the great interest in the spider silk mechanical properties, targeting its use in biomedical and biotechnological applications due to its properties of strength...

Structure and post-translational modifications of the web silk protein spidroin-1 from Nephila spiders

Aparecido dos Santos-Pinto, Jose Roberto; Lamprecht, Guenther; Chen, Wei-Qiang; Heo, Seok; Hardy, John George; Priewalder, Helga; Scheibel, Thomas Rainer; Palma, Mario Sergio; Lubec, Gert
Fonte: Elsevier B.V. Publicador: Elsevier B.V.
Tipo: Artigo de Revista Científica Formato: 174-185
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Processo FAPESP: 10/19051-6; Processo FAPESP: 11/51684-1; Spidroin-1 is one of the major ampullate silk proteins produced by spiders for use in the construction of the frame and radii of orb webs, and as a dragline to escape from predators. Only partial sequences of spidroin-1 produced by Nephila clavipes have been reported up to now, and there is no information on post-translational modifications (PTMs). A gel-based mass spectrometry strategy with ETD and CID fragmentation methods were used to sequence and determine the presence/location of any PTMs on the spidroin-1. Sequence coverage of 98.06%, 95.05%, and 98.37% were obtained for N. clavipes, Nephila edulis and for Nephila madagascariensis, respectively. Phosphorylation was the major PTM observed with 8 phosphorylation sites considered reliable on spidroin-1 produced by N. clavipes, 4 in N. madagascariensis and 2 for N. edulis. Dityrosine and 3,4-dihydroxyphenylalanine (formed by oxidation of the spidroin-1) were observed, although the mechanism by which they are formed (i.e. exposure to UV radiation or to peroxidases in the major ampullate silk gland) is uncertain. Herein we present structural information on the spidroin-1 produced by three different Nephila species; these findings may be valuable for understanding the physicochemical properties of the silk proteins and moreover...

Avaliação histométrica do efeito do transplante autógeno de células do ligamento periodontal no tratamento de defeitos de furca grau III em cães; Autologous periodontal ligament cells in the treatment of class III furcation defects : a histometric study in beagle dogs

Fabricia Ferreira Suaid
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 28/04/2010 Português
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O objetivo do presente estudo foi avaliar histometricamente o efeito dotransplante autógeno de células do ligamento periodontal (PDLCs), associado à regeneração tecidual guiada (RTG), no tratamento de defeitos de furca grau III criados cirurgicamente em cães. Inicialmente, as PDLCs foram obtidas das raízes do 2º pré-molar e do 1º molar inferior extraídos, bilateralmente, de sete cães da raça beagle. Em seguida, as células foram cultivadas in vitro e caracterizadas fenotipicamente. Lesões de furca grau III foram criadas nos 3os e 4os pré-molares inferiores e os defeitos foram aleatoriamente escolhidos para receber os seguintes tratamentos: Grupo Controle – instrumentação da superfície radicular com auxílio de curetas e posicionamento coronário dos retalhos (7); Grupo RTG – regeneração tecidual guiada (7); Grupo Esponja – RTG + esponja de colágeno (7); Grupo Células – RTG + células do ligamento periodontal embebidas na esponja de colágeno, na ausência de soro fetal bovino (7). Após três meses, os animais foram sacrificados e os blocos contendo os espécimes foram processados para análise histológica. Os parâmetros histométricos avaliados foram: extensão total do defeito (ETD), extensão não preenchida do defeito (ENP)...

3D Surface Profile Equipment for the Characterization of the Pavement Texture - TexScan

Vilaça, João L.; Fonseca, Jaime C.; Pinho, A. C. Marques de; Freitas, Elisabete F.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Publicado em 08/07/2010 Português
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Loads from vehicles alter the functional and structural characteristics of road pavements that directly affect the loss of resistance of the pavement and the users’ comfort and safety. Those alterations require constant observation and analysis of an extensive area of road surface with high precision. For such it was developed a new scanning prototype machine capable of acquiring the 3D road surface data and characterize the road texture through two algorithms that allows calculate the Estimated Texture Depth (ETD) and Texture Profile Level (L) indicators. The experimental results obtained from nine road samples validate the developed algorithms for the texture analysis and showed good agreement between the scanning prototype equipment and the traditional Sand Patch Method.; Fundação para a Ciência e a Tecnologia (FCT) through the PhD Grant referenced SFRH/ BD/18155/2004

Cloning and Sequence Analysis of Genes Encoding Staphylococcus hyicus Exfoliative Toxin Types A, B, C, and D

Ahrens, Peter; Andresen, Lars Ole
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /03/2004 Português
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Exfoliative toxins produced by certain strains of Staphylococcus hyicus mediate exudative epidermitis in pigs. In this study the genes coding for four different exfoliative toxin from S. hyicus (ExhA, ExhB, ExhC, and ExhD) were cloned and sequenced. The coding sequence of the four toxin genes ranged from 816 to 834 bp. The amino acid sequences of these four toxins were homologous to the earlier described exfoliative toxins SHETB from S. hyicus and ETA, ETB, and ETD from Staphylococcus aureus. The homology between the S. hyicus toxins was at the same level as the homology to the exfoliative toxins from S. aureus. The toxins showed similarity to serine proteases, including preservation of the catalytic tract in ExhA, ExhB, and ExhC. However, in ExhD, Asp in the putative catalytic tract was replaced with Glu. The recombinant toxins could be expressed in Escherichia coli, and three of the four toxins were recognized by monoclonal antibodies raised against native exfoliative toxins.

Staphylococcus aureus Isolates Carrying Panton-Valentine Leucocidin Genes in England and Wales: Frequency, Characterization, and Association with Clinical Disease

Holmes, A.; Ganner, M.; McGuane, S.; Pitt, T. L.; Cookson, B. D.; Kearns, A. M.
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /05/2005 Português
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Staphylococcus aureus isolates carrying the genes that encode for Panton-Valentine leucocidin (PVL), a highly potent toxin, have been responsible for recent outbreaks of severe invasive disease in previously healthy children and adults in the United States of America and Europe. To determine the frequency of PVL-positive isolates sent to the Staphylococcus Reference Unit (United Kingdom) for epidemiological purposes, we tested 515 isolates of S. aureus, and 8 (1.6%) were positive for the PVL locus. A further 470 isolates were selected to explore the association of PVL-positive S. aureus with clinical disease. Of these, 23 (4.9%) were PVL positive and most were associated with skin and soft tissue infections (especially abscesses). The PVL genes were also detected in isolates responsible for community-acquired pneumonia, burn infections, bacteremia, and scalded skin syndrome. Genotyping by pulsed-field gel electrophoresis and multilocus sequence typing revealed that the PVL-positive isolates were from diverse genetic backgrounds, although one prevalent clone of 12 geographically dispersed methicillin-resistant S. aureus (MRSA) isolates was identified (ST80). All 12 isolates were stapylococcal cassette chromosome mec type IVc, had an agr3 allele...

Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry

Chi, An; Huttenhower, Curtis; Geer, Lewis Y.; Coon, Joshua J.; Syka, John E. P.; Bai, Dina L.; Shabanowitz, Jeffrey; Burke, Daniel J.; Troyanskaya, Olga G.; Hunt, Donald F.
Fonte: National Academy of Sciences Publicador: National Academy of Sciences
Tipo: Artigo de Revista Científica
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We present a strategy for the analysis of the yeast phosphoproteome that uses endo-Lys C as the proteolytic enzyme, immobilized metal affinity chromatography for phosphopeptide enrichment, a 90-min nanoflow-HPLC/electrospray-ionization MS/MS experiment for phosphopeptide fractionation and detection, gas phase ion/ion chemistry, electron transfer dissociation for peptide fragmentation, and the Open Mass Spectrometry Search Algorithm for phosphoprotein identification and assignment of phosphorylation sites. From a 30-μg (≈600 pmol) sample of total yeast protein, we identify 1,252 phosphorylation sites on 629 proteins. Identified phosphoproteins have expression levels that range from <50 to 1,200,000 copies per cell and are encoded by genes involved in a wide variety of cellular processes. We identify a consensus site that likely represents a motif for one or more uncharacterized kinases and show that yeast kinases, themselves, contain a disproportionately large number of phosphorylation sites. Detection of a pHis containing peptide from the yeast protein, Cdc10, suggests an unexpected role for histidine phosphorylation in septin biology. From diverse functional genomics data, we show that phosphoproteins have a higher number of interactions than an average protein and interact with each other more than with a random protein. They are also likely to be conserved across large evolutionary distances.

P71-M Identification of Glycosylation Sites Using Electron Transfer Dissociation in a Linear Ion Trap

Huang, Y.; Hao, Z.; Twine, S.; Mullen, J.; Waddell, K.; Kelly, J.
Fonte: The Association of Biomolecular Resource Facilities Publicador: The Association of Biomolecular Resource Facilities
Tipo: Artigo de Revista Científica
Publicado em /02/2007 Português
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Glycosylation of proteins plays an important role in biological systems. The attachment of polysaccharide chains serves various key cellular functions. Thus, it is often critical to determine the exact site of glycosylation. Tandem mass spectrometry (MS/MS) employing linear ion trap technology has emerged as a cornerstone for protein identification and PTM analysis in bottom-up proteomics. However, the glycosylation moiety is usually fragile and falls off easily during the collision-induced dissociation (CID) MS/ MS activation process without leaving much evidence of its sites of attachment. Electron transfer dissociation (ETD) has recently been commercially introduced onto a linear ion trap. This new fragmentation methodology shows great promise in its ability to preserve the saccharide side chains on a peptide, while obtaining fragmentation between the α-carbon and the nitrogen throughout the backbone. This enables the exact glycosylation site to be deduced from the MS/MS spectrum.

P96-T Characterization the Sequence and Post-Translational Modification of Large Peptides by Tandem ETD MS/MS

Brekenfeld, A.; Bäßmann, C.; Hartmer, R.
Fonte: The Association of Biomolecular Resource Facilities Publicador: The Association of Biomolecular Resource Facilities
Tipo: Artigo de Revista Científica
Publicado em /02/2007 Português
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Protein/peptide analysis is commonly based on use of a tandem mass spectrometer combining electrospray ionization and collision-induced dissociation (CID). The major disadvantage of collisional ion activation is the internal heating of the parent ion, which predominantly yields cleavages of the weakest bonds, resulting in less informative fragment spectra; this often limits peptide sequence determination.

P145-S Interactive and Automated Top-Down Analysis of Sequencing Data of Intact Proteins

Suckau, D.; Vorwerg, L.; Resemann, A.; Witt, M.; Easterling, M.
Fonte: The Association of Biomolecular Resource Facilities Publicador: The Association of Biomolecular Resource Facilities
Tipo: Artigo de Revista Científica
Publicado em /02/2007 Português
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In a Top-Down workflow (ECD, ETD, ISD) the whole protein molecule is subjected to fragmentation and proteins are not digested (as in the Bottom-Up approach). This allows the detection of signal peptides, modifications, sequence variations and mutations. Top-Down analyses can be done using FTMS/ECD/ESI or MALDI-ISD spectra.

Ion Trap Collisional Activation of c and z• Ions Formed via Gas-Phase Ion/Ion Electron Transfer Dissociation

Han, Hongling; Xia, Yu; McLuckey, Scott A.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
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A series of c- and z•-type product ions formed via gas-phase electron transfer ion/ion reactions between protonated polypeptides with azobenzene radical anions are subjected to ion trap collision activation in a linear ion trap. Fragment ions including a-, b-, y-type and ammonia-loss ions are typically observed in collision induced dissociation (CID) of c ions, showing almost identical CID patterns as those of the C-terminal amidated peptides consisting of the same sequences. Collisional activation of z• species mainly gives rise to side-chain losses and peptide backbone cleavages resulting in a-, b-, c-, x-, y-and z-type ions. Most of the fragmentation pathways of z• species upon ion trap CID can be accounted for by radical driven processes. The side-chain losses from z• species are different from the small losses observed from the charge-reduced peptide molecular species in electron transfer dissociation (ETD), which indicates rearrangement of the radical species. Characteristic side-chain losses are observed for several amino acid residues, which are useful to predict their presence in peptide/protein ions. Furthermore, the unique side-chain losses from leucine and isoleucine residues allow facile distinction of these two isomeric residues.

Implementation of Ion/Ion Reactions in a Quadrupole/Time-of-Flight Tandem Mass Spectrometer

Xia, Yu; Chrisman, Paul A.; Erickson, David E.; Liu, Jian; Liang, Xiaorong; Londry, Frank A.; Yang, Min J.; McLuckey, Scott A.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em 15/06/2006 Português
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A commercial quadrupole/time-of-flight (QqTOF) tandem mass spectrometer has been adapted for ion/ion reaction studies. To enable mutual storage of oppositely charged ions in a linear ion trap, the oscillating quadrupole field of the second quadrupole of the system (Q2) serves to store ions in the radial dimension while auxiliary RF is superposed on the end lenses of Q2 during the reaction period to create barriers in the axial dimension. A pulsed dual electrospray (ESI) source is directly coupled to the instrument interface for the purpose of proton transfer reactions. Singly and doubly charged protein ions as high in mass as 66 kDa are readily formed and observed after proton transfer reactions. For the modified instrument, the mass resolving power is about 8000 for a wide m/z range and the mass accuracy is ~20 ppm for external calibration and ~5 ppm for internal calibration after ion/ion reactions. Parallel ion parking is demonstrated with a six-component protein mixture, which shows the potential application of reducing spectral complexity and concentrating certain charge states. The current system has high flexibility with respect to defining MSn experiments involving collision-induced dissociation (CID) and ion/ion reactions. Protein precursor and CID product masses can be determined with good accuracy...

Microemulsion-Based Vaginal Gel of Clotrimazole: Formulation, In Vitro Evaluation, and Stability Studies

Bachhav, Yogeshwar G.; Patravale, Vandana B.
Fonte: Springer US Publicador: Springer US
Tipo: Artigo de Revista Científica
Publicado em 21/04/2009 Português
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The objective of the present investigation was to develop and evaluate microemulsion-based gel for the vaginal delivery of clotrimazole (CMZ). The solubility of CMZ in oils and surfactants was evaluated to identify components of the microemulsion. The ternary diagram was plotted to identify the area of microemulsion existence. Various gelling agents were evaluated for their potential to gel the CMZ microemulsion without affecting its structure. The bioadhesive potential and antifungal activity of the CMZ microemulsion-based gel (CMZ-MBG) was determined in comparison to the marketed clotrimazole gel (Candid-V® gel) by in vitro methods. The chemical stability of CMZ in CMZ-MBG was determined as per the International Conference on Harmonization guidelines. The CMZ microemulsion exhibited globule size of 48.4 nm and polydispersity index of 0.75. Carbopol® ETD 2020 could successfully gel the CMZ microemulsion without disturbing the structure. The CMZ-MBG showed significantly higher (P < 0.05) in vitro bioadhesion and antifungal activity as compared to that of Candid-V® gel. The stability studies indicated that CMZ undergoes acidic pH-catalyzed degradation at all the storage conditions at the end of 3 months.

Variation in Antagonism of the Interferon Response to Rotavirus NSP1 Results in Differential Infectivity in Mouse Embryonic Fibroblasts▿

Feng, N.; Sen, A.; Nguyen, H.; Vo, P.; Hoshino, Y.; Deal, E. M.; Greenberg, H. B.
Fonte: American Society for Microbiology (ASM) Publicador: American Society for Microbiology (ASM)
Tipo: Artigo de Revista Científica
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Rotavirus NSP1 has been shown to function as an E3 ubiquitin ligase that mediates proteasome-dependent degradation of interferon (IFN) regulatory factors (IRF), including IRF3, -5, and -7, and suppresses the cellular type I IFN response. However, the effect of rotavirus NSP1 on viral replication is not well defined. Prior studies used genetic analysis of selected reassortants to link NSP1 with host range restriction in the mouse, suggesting that homologous and heterologous rotaviruses might use their different abilities to antagonize the IFN response as the basis of their host tropisms. Using a mouse embryonic fibroblast (MEF) model, we demonstrate that heterologous bovine (UK and NCDV) and porcine (OSU) rotaviruses fail to effectively degrade cellular IRF3, resulting in IRF3 activation and beta IFN (IFN-β) secretion. As a consequence of this failure, replication of these viruses is severely restricted in IFN-competent wild-type, but not in IFN-deficient (IFN-α/β/γ receptor- or STAT1-deficient) MEFs. On the other hand, homologous murine rotaviruses (ETD or EHP) or the heterologous simian rotavirus (rhesus rotavirus [RRV]) efficiently degrade cellular IRF3, diminish IRF3 activation and IFN-β secretion and are not replication restricted in wild-type MEFs. Genetic reassortant analysis between UK and RRV maps the distinctive phenotypes of IFN antagonism and growth restriction in wild-type MEFs to NSP1. Therefore...

Peptide Fragmentation Induced by Radicals at Atmospheric Pressure

Vilkov, Andrey N.; Laiko, Victor V.; Doroshenko, Vladimir M.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /04/2009 Português
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A novel ion dissociation technique, which is capable of providing an efficient fragmentation of peptides at essentially atmospheric pressure conditions, is developed. The fragmentation patterns observed often contain c-type fragments that are specific to ECD/ETD, along with the y-/b- fragments that are specific to CAD. In the presented experimental setup, ion fragmentation takes place within a flow reactor located in the atmospheric pressure region between the ion source and the mass spectrometer. According to a proposed mechanism, the fragmentation results from the interaction of ESI-generated analyte ions with the gas-phase radical species produced by a corona discharge source.

Clonal Complexes and Diversity of Exotoxin Gene Profiles in Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus Isolates from Patients in a Spanish Hospital▿

Argudín, M. A.; Mendoza, M. C.; Méndez, F. J.; Martín, M. C.; Guerra, B.; Rodicio, M. R.
Fonte: American Society for Microbiology (ASM) Publicador: American Society for Microbiology (ASM)
Tipo: Artigo de Revista Científica
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Molecular epidemiology studies have allowed the identification of the methicillin (meticillin)-resistant (MRSA) and methicillin-susceptible (MSSA) clonal complexes (CCs) and clones of Staphylococcus aureus circulating in a Spanish hospital recently. Of 81 isolates tested, 32.1% were MRSA. Most of them carried staphylococcal cassette chromosome mec (SCCmec) IVc (88.5%) and belonged to CC5 (88.5%; multilocus sequence typing types ST125 [mainly associated with spa type t067], ST5, and ST228). A higher diversity was found among MSSA isolates (67.9%). Eighty percent shared the genetic background of major MRSA lineages (CC5 [38.2%; ST125 and ST5], CC30 [25.5%; ST30], CC45 [14.5%; ST45 and ST47], and CC8 [1.8%; ST8]), but CC12, CC15, CC51, and CC59 were also detected. Many exotoxin genes were present in each of the 81 isolates, independent of whether they were involved in sepsis (11 to 22) or other types of infections (13 to 21), and they appeared in 73 combinations. The relevant data are that (i) all isolates were positive for hemolysin and leukotoxin genes (98.8% for lukED and 25.9% for lukPV); (ii) all contained an enterotoxin gene cluster (egc with or without seu), frequently with one or more genes encoding classical enterotoxins; (iii) about half were positive for tst and 95% were positive for exfoliatin-encoding genes (eta...

A Straightforward and Highly Efficient Precipitation/On-pellet Digestion Procedure Coupled to a Long Gradient Nano-LC Separation and Orbitrap Mass Spectrometry for Label-free Expression Profiling of the Swine Heart Mitochondrial Proteome

Duan, Xiaotao; Young, Rebecca; Straubinger, Robert M.; Page, Brian J.; Cao, Jin; Wang, Hao; Yu, Haoying; Canty, John M.; Qu, Jun
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /06/2009 Português
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For label-free expression profiling of tissue proteomes, efficient protein extraction, thorough and quantitative sample cleanup and digestion procedures, as well as sufficient and reproducible chromatographic separation, are highly desirable but remain challenging. However, optimal methodology has remained elusive, especially for proteomes that are rich in membrane proteins, such as the mitochondria. Here we describe a straightforward and reproducible sample preparation procedure, coupled with a highly selective and sensitive nano-LC/Orbitrap analysis, which enables reliable and comprehensive expression profiling of tissue mitochondria. The mitochondrial proteome of swine heart was selected as a test system. Efficient protein extraction was accomplished using a strong buffer containing both ionic and non-ionic detergents. Overnight precipitation was used for cleanup of the extract, and the sample was subjected to an optimized 2-step, on-pellet digestion approach. In the first step, the protein pellet was dissolved via a 4 h tryptic digestion under vigorous agitation, which nano-LC/LTQ/ETD showed to produce large and incompletely cleaved tryptic peptides. The mixture was then reduced, alkylated, and digested into its full complement of tryptic peptides with additional trypsin. This solvent precipitation/on-pellet digestion procedure achieved significantly higher and more reproducible peptide recovery of the mitochondrial preparation...

Improved Methods for the Enrichment and Analysis of Glycated Peptides

Zhang, Qibin; Schepmoes, Athena A.; Brock, Jonathan W. C.; Wu, Si; Moore, Ronald J.; Purvine, Samuel O.; Baynes, John W.; Smith, Richard D.; Metz, Thomas O.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em 15/12/2008 Português
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Nonenzymatic glycation of tissue proteins has important implications in the development of complications of diabetes mellitus. Herein we report improved methods for the enrichment and analysis of glycated peptides using boronate affinity chromatography and electron-transfer dissociation mass spectrometry, respectively. The enrichment of glycated peptides was improved by replacing an off-line desalting step with an online wash of column-bound glycated peptides using 50 mM ammonium acetate, followed by elution with 100 mM acetic acid. The analysis of glycated peptides by MS/MS was improved by considering only higher charged (≥3) precursor ions during data-dependent acquisition, which increased the number of glycated peptide identifications. Similarly, the use of supplemental collisional activation after electron transfer (ETcaD) resulted in more glycated peptide identifications when the MS survey scan was acquired with enhanced resolution. Acquiring ETD-MS/MS data at a normal MS survey scan rate, in conjunction with the rejection of both 1+ and 2+ precursor ions, increased the number of identified glycated peptides relative to ETcaD or the enhanced MS survey scan rate. Finally, an evaluation of trypsin, Arg-C, and Lys-C showed that tryptic digestion of glycated proteins was comparable to digestion with Lys-C and that both were better than Arg-C in terms of the number of glycated peptides and corresponding glycated proteins identified by LC–MS/MS.

Wrist Innovations To Improve Function of Electric Terminal Devices

Sears, Harold H.; Iversen, Edwin; Archer, Shawn; Jacobs, Tony
Fonte: Myoelectric Symposium Publicador: Myoelectric Symposium
Tipo: Artigo de Revista Científica
Publicado em //2008 Português
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A major challenge in the development of terminal device (TD) and UE prosthetic devices is to add to the functional benefits to the wearer, without greatly increasing the weight, or complexity, or the cost of the prosthesis. Using existing TD designs, the opportunity existed to increase the function by increasing the degree of freedom available at the wrist, in several ways. Since the existing hands and electric terminal devices (ETD) were both single degree-of-freedom TDs, improving the positionability of the TD can logically improve the gripping orientation and grip security. Our goal was therefore, to improve positionability of TDs via improved wrist flexion/extension devices, and an improved wrist rotation device, which could be added in a modular fashion to the existing MC Hand, and ETD already developed and used extensively in the field.

Adjusting the margins: Building bridges between deaf and hearing cultures through performance arts

Davis Haggerty, Luane
Fonte: Rochester Instituto de Tecnologia Publicador: Rochester Instituto de Tecnologia
Tipo: Dissertação
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This study addresses a gap in scholarship on leadership styles in the Deaf community. There is an invisible style of leadership differing from the mainstream culture that has not been previously addressed in the literature at any depth. My study was composed of three interlocking parts in a sequence that constitutes the practice of anthropology: fieldwork, analysis, and presentation. The foundation for my fieldwork was an “archeology of the structure of the perceived world” (Merleau-Ponty), using the holding environment of the rehearsal process and the structural process of an acting technique called Del-Sign. Del- Sign is a fusion acting style that I created by combining American Sign Language and the Delsarte method. I also employed current qualitative methods described as “performance ethnography” (Norman Denzin and Ron Pelias). The fieldwork of creating discussion groups, which I call salons, provided the initial material, my analysis process turned that material into a performance script; and audience participation in the form of talk-back sessions after the performance provided documentation for the results of the presentation. I provided data for the fieldwork with journaling and videotaping events in rehearsals and performances...