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Nutritional and antioxidant contributions of Laurus nobilis L. leaves: would be more suitable a wild or a cultivated sample?

Dias, Maria Inês; Barros, Lillian; Dueñas, Montserrat; Alves, Rita C.; Oliveira, M.B.P.P.; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Português
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Medicinal and aromatic plants are used since ancient times in folk medicine and traditional food, but also in novel pharmaceutical preparations. The controversy lies in the use of cultivated and/or wild plants presenting both advantages and disadvantages in biological, ecological but also economic terms. Herein, cultivated and wild samples of Laurus nobilis L. were chemically characterized regarding nutritional value, free sugars, organic acids, fatty acids and tocopherols. Furthermore, the antioxidant activity (scavenging activity, reducing power and lipid peroxidation inhibition) and individual phenolic profile of L. nobilis extracts and infusions were evaluated. Data showed that the wild sample gave higher nutritional contribution related to a higher content of proteins, free sugars, organic acids, PUFA and tocopherols. It also gave better PUFA/SFA and n − 6/n − 3 ratios. Regarding antioxidant activity and phenolic compounds, it was the cultivated sample (mostly the infusion) that showed the highest values. The present study supports the arguments defending the use of wild and cultivated medicinal and aromatic plants as both present very interesting features, whether nutritional or antioxidant, that can be an assessed by their consumption. In vitro culture could be applied to L. nobilis as a production methodology that allows combination of the benefits of wild and cultivated samples.

Effects of gamma irradiation on macro and microelements of Boletus edulis Bull.: Fr. and Hydnum repandum L.: Fr.

Fernandes, Amilcar L.; Barreira, João C.M.; Antonio, Amilcar L.; Morales, Patricia; Fernández-Ruiz, Virginia; Oliveira, M.B.P.P.; Martins, Anabela; Ferreira, Isabel C.F.R.
Fonte: Universidade do Minho Publicador: Universidade do Minho
Tipo: Conferência ou Objeto de Conferência
Português
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Mushrooms are considered excellent sources of minerals, being necessary to know the levels of essential elements in the edible species [1]. Mineral elements are classified in macro (e.g., Ca, Mg, Na, K) and microelements (e.g., Fe, Cu, Mn, Zn) with functions that include maintaining acid-base balance, osmotic regulation, oxygen transport and enzymatic cofactors [2-3]. Mushrooms need special caution in their conservation. Accordingly, treatments such as gamma rays have been applied to improve their shelf life and reduce health hazards caused by pathogenic microorganisms [4]. In this work, gamma irradiation was applied to Boletus edulis Bull.: Fr. and Hydnum repandum L.: Fr. to assess the effects on the minerals composition. The fruiting bodies were collected in Trás-os-Montes (Northeast of Portugal) in November 2012. Fresh samples were irradiated in experimental equipment with four 60Co sources, at 1 and 2 kGy. The studied mushrooms presented high levels of macro and microelements. Up to the applied doses, some significant differences were observed. However, in most cases, changes did not follow a marked tendency, being more likely to be derived from the natural variability in mineral accumulation as a result of mushroom growth in different ecosystems. Hence...

Association of the UGT1A1-53(TA)(n) polymorphism with L-thyroxine doses required for thyrotropin suppression in patients with differentiated thyroid cancer

VARGENS, Daniela D.; NEVES, Ronaldo R. S.; BULZICO, Daniel A.; OJOPI, Elida B.; MEIRELLES, Ricardo M. R.; PESSOA, Cencita N.; PRADO, Carolina M.; GONCALVES, Pedro A.; LEAL, Vera L. G.; SUAREZ-KURTZ, Guilherme
Fonte: LIPPINCOTT WILLIAMS & WILKINS Publicador: LIPPINCOTT WILLIAMS & WILKINS
Tipo: Artigo de Revista Científica
Português
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There is a considerable interindividual variation in L-thyroxine [ 3,5,3`,5`-tetraiodo-l-thyronine (T(4))] dose required for thyrotropin (thyroid-stimulating hormone) suppression in patients with differentiated thyroid cancer. To investigate whether uridine diphosphate-glucuronosyl transferase 1A1 (UGT1A1)-mediated T(4) glucuronidation in liver affects T(4) dose, we genotyped 101 patients for the common UGT1A1-53(TA)(n) polymorphism and compared T(4) doses among patients having zero (5/6 and 6/6 genotypes), one (6/7 genotype), or two (7/7 and 7/8 genotypes) copies of the low-expression (TA) 7 and (TA) 8 alleles. A significant trend for decreasing T(4) dose with increasing number of copies of (TA)(7) and (TA)(8) (P = 0.037) and significant difference in T(4) dose across the UGT1A1-53(TA)(n) genotypes (P = 0.048) were observed, despite considerable overlap of T(4) doses among different genotypes. These results are consistent with reduced T(4) glucuronidation in patients with low-expression (TA) 7 and (TA) 8 alleles and provide the first evidence for association between UGT1A1-53(TA)(n) and T(4)-dose requirement for thyroid-stimulating hormone suppression in a natural clinical setting. Pharmacogenetics and Genomics 21: 341-343 (C) 2011 Wolters Kluwer Health | Lippincott Williams & Wilkins. Pharmacogenetics and Genomics 2011...

H-1-N-15 NMR studies of the complex bis(S-allyl-L-cysteinate)palladium(II)

Corbi, P. P.; Massabni, A. C.
Fonte: Elsevier B.V. Publicador: Elsevier B.V.
Tipo: Artigo de Revista Científica Formato: 418-419
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H-1-N-15 2D NMR data for S-allyl-L-cysteine (deoxyalliin) and for bis(S-allyl-L-cysteinate)palladium(II) complex are presented in this manuscript. Large upfield N-15 NMR shift of the amine nitrogen in the spectrum of the complex when compared to the spectrum of the ligand shows clearly coordination of S-allyl-L-cysteine, in the anion form, to palladium(II) through the NH2 group. (c) 2005 Elsevier B.V. All rights reserved.

Detection of P. aeruginosa harboring bla(CTX-M-2), bla(GES-1) and bla(GES-5), bla(IMP-1) and bla(SPM-1) causing infections in Brazilian tertiary-care hospital

Polotto, Milena; Casella, Tiago; de Lucca Oliveira, Maria Gabriela; Rubio, Fernando G.; Nogueira, Mauricio L.; de Almeida, Margarete T. G.; Nogueira, Mara C. L.
Fonte: Biomed Central Ltd. Publicador: Biomed Central Ltd.
Tipo: Artigo de Revista Científica Formato: 8
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Background: Nosocomial infections caused by Pseudomonas aeruginosa presenting resistance to beta-lactam drugs are one of the most challenging targets for antimicrobial therapy, leading to substantial increase in mortality rates in hospitals worldwide. In this context, P. aeruginosa harboring acquired mechanisms of resistance, such as production of metallo-beta-lactamase (MBLs) and extended-spectrum beta-lactamases (ESBLs) have the highest clinical impact. Hence, this study was designed to investigate the presence of genes codifying for MBLs and ESBLs among carbapenem resistant P. aeruginosa isolated in a Brazilian 720-bed teaching tertiary care hospital.Methods: Fifty-six carbapenem-resistant P. aeruginosa strains were evaluated for the presence of MBL and ESBL genes. Strains presenting MBL and/or ESBL genes were submitted to pulsed-field gel electrophoresis for genetic similarity evaluation.Results: Despite the carbapenem resistance, genes for MBLs (bla(SPM-1) or bla(IMP-1)) were detected in only 26.7% of isolates. Genes encoding ESBLs were detected in 23.2% of isolates. The bla(CTX-M-2) was the most prevalent ESBL gene (19.6%)...

L-735,524: an orally bioavailable human immunodeficiency virus type 1 protease inhibitor.

Vacca, J P; Dorsey, B D; Schleif, W A; Levin, R B; McDaniel, S L; Darke, P L; Zugay, J; Quintero, J C; Blahy, O M; Roth, E
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em 26/04/1994 Português
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To date, numerous inhibitors of the human immunodeficiency virus type 1 protease have been reported, but few have been studied extensively in humans, primarily as a consequence of poor oral bioavailability in animal models. L-735,524 represents a class of human immunodeficiency virus type 1 protease inhibitors, termed hydroxyaminopentane amides, that incorporate a basic amine into the hydroxyethylene inhibitor backbone. L-735,524 is a potent inhibitor of virus replication in cell culture and inhibits the protease-mediated cleavage of the viral precursor polyproteins that results in the production of noninfectious progeny viral particles. The compound is effective against viruses resistant to reverse transcriptase inhibitors and is synergistically active when used in combination with reverse transcriptase inhibitors. Most importantly, L-735,524 exhibits good oral bioavailability and plasma pharmacokinetic profiles in two species of laboratory animals by using clinically acceptable formulations. Accordingly, the compound was selected for evaluation of safety and pharmacokinetic studies in humans.

Inhibition of human immunodeficiency virus integrase by bis-catechols.

LaFemina, R L; Graham, P L; LeGrow, K; Hastings, J C; Wolfe, A; Young, S D; Emini, E A; Hazuda, D J
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /02/1995 Português
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The human immunodeficiency virus type 1 (HIV-1) integrase protein is required for the productive infection of T-lymphoid cells in culture (R. L. LaFemina, C. L. Schneider, H. L. Robbins, P. L. Callahan, K. LeGrow, E. Roth, W. A. Schleif, and E. A. Emini, J. Virol. 66:7414-7419, 1992). This observation suggests that chemical inhibitors of integrase may prevent the spread of HIV in infected individuals. In our search for such potential chemotherapeutic agents, we observed that beta-conidendrol inhibits both the sequence-dependent and sequence-independent endonucleolytic activities of integrase with comparable potencies in vitro (50% inhibitory concentration, 500 nM). Structurally related compounds tested for their abilities to inhibit integrase generated a limited structure-activity analysis which demonstrated that potency is associated with the bis-catechol structure: two pairs of adjacent hydroxyls on separate benzene rings. beta-Conidendrol did not inhibit several other endonucleases and/or phosphoryltransferases. Although beta-conidendrol was not effective in preventing HIV-1 infection in cell culture, the in vitro data demonstrate that it is possible to identify selective agents targeted against this essential HIV-1 function.

The effects of the cyclosporin A, a P-glycoprotein inhibitor, on the pharmacokinetics of baicalein in the rat: a microdialysis study

Tsai, T H; Liu, S C; Tsai, P L; Ho, L K; Shum, A Y C; Chen, C F
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
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Baicalein is a bioactive flavonoid isolated from the root of Scutellaria baicalensis Georgi, a medicinal herb that has been used since ancient times to treat bacterial infections. As little is known concerning its pharmacokinetics, this study focussed on its pharmacokinetics as well as the possible roles of the multidrug transporter P-glycoprotein on its distribution and disposition.Three microdialysis probes were simultaneously inserted into the jugular vein, the hippocampus and the bile duct of male Sprague–Dawley rats for sampling in biological fluids following the administration of baicalein (10, 30 and 60 mg kg−1) through the femoral vein. The P-glycoprotein inhibitor cyclosporin A was used to help delineate its roles.The study design consisted of two groups of six rats in parallel: control rats which received baicalein alone and the cyclosporin A treated-group in which the rats were injected cyclosporin A, a P-glycoprotein inhibitor, 10 min prior to baicalein administration.Cyclosporin A treatment resulted in a significant increase in elimination half-life, mean residence time and area under the concentration versus time curve (AUC) of unbound baicalein in the brain. However, AUC in the bile was decreased.The decline of baicalein in the hippocampus...

Heranca da estatura de planta e do comprimento da panicula principal no cruzamento entre Avena sativa L. e Avena sterilis L.

MARIOT, M.P.; SERENO, M.J.C. de M.; FEDERIZZI, L.C.; CARVALHO, F.I.F. de
Fonte: Pesquisa Agropecuaria Brasileira, Brasilia, v.34, n.1, p.77-82, jan. 1999. Publicador: Pesquisa Agropecuaria Brasileira, Brasilia, v.34, n.1, p.77-82, jan. 1999.
Tipo: Artigo em periódico indexado (ALICE)
Português
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A especie selvagem Avena sterilis L. tem sido amplamente utilizada para cruzamento com aveia cultivada, pela alta afinidade existente entre ambas, o que possibilita um aumento na variabilidade genetica e transferencia de genes de importancia agronomica. Entretanto, caracteres indesejaveis, como a alta estatura e um maior comprimento de panicula, podem ser transferidos a progenie. Nesse sentido, com o objetivo de avaliar a variabilidade genetica, bem como a heranca em cruzamentos entre Avena sativa L. e Avena sterilis L., foram mensurados os caracteres estatura de planta e comprimento da panicula principal. As variancias fenotipicas e geneticas foram altas e as variancias de ambiente foram baixas, proporcionando uma alta herdabilidade no sentido amplo. O efeito genio de aditividade foi o mais importante para explicar a variacao genetica em ambos os caracteres.; 1999

Phosphate solubilization and synergism between P-solubilizing and arbuscular mycorrhizal fungi.

SOUCHIE, E.L.; AZCÓN, R.; BAREA, J.M.; SAGGIN-JÚNIOR, O.J.; SILVA, E.M.R. da
Fonte: Pesquisa Agropecuária Brasileira, Brasília, v.41, n. 9, p. 1405-1411, set. 2006. Publicador: Pesquisa Agropecuária Brasileira, Brasília, v.41, n. 9, p. 1405-1411, set. 2006.
Tipo: Artigo em periódico indexado (ALICE)
Português
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The objective of this work was to evaluate the ability of several P-solubilizing fungi to solubilize aluminum phosphate and Araxá apatite as well as the synergism between the P-solubilizing fungus, PSF 7, and arbuscular mycorrhizal fungi to promote clover growth amended with aluminum phosphate. Two experiments were carried out, the first under laboratory conditions and the second in a controlled environmental chamber. In the first experiment, PSF 7, PSF 9, PSF 21 and PSF 22 isolates plus control were incubated in liquid medium at 28ºC for eight days. On the 2nd, 4th and 8th day of incubation, pH and soluble P were determined. In the second experiment, clover was sowed in plastic pots containing 300 g of sterilized substrate amended with aluminum phosphate, 3 g L-1, in presence and absence of PSF 7 isolate and arbuscular mycorrhizal fungi. A completely randomized design, in factorial outline 2x2 (presence and absence of PSF 7 and arbuscular mycorrhizal fungi) and five replicates were used. In the first experiment, higher P content was detected in the medium containing aluminum phosphate. PSF 7 is the best fungi isolate which increases aluminum solubilization with major tolerance to Al3+. Clover growth was stimulated by presence of PSF 7 and arbuscular mycorrhizal fungi. There is synergism between microorganisms utilized to improve plant nutrition.; 2006

Enzyme activities and pectin breakdown of sapodilla submitted to 1-methylcyclopropene.

MORAIS, P.L.D. de; LIMA, L.C.
Fonte: Pesquisa Agropecuária Brasileira, Brasília, DF, v.43, n.1, p.15-20, jan. 2008. Publicador: Pesquisa Agropecuária Brasileira, Brasília, DF, v.43, n.1, p.15-20, jan. 2008.
Tipo: Artigo em periódico indexado (ALICE)
Português
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The objective of this work was to investigate the influence of 1-methylcyclopropene (1-MCP) at 300 nL L-1 on activities of cell wall hidrolytic enzymes and pectin breakdown changes which Sapodilla (Manilkara zapota cv. Itapirema 31) cell wall undergoes during ripening. Sapodilla were treated with ethylene antagonist 1-MCP at 300 nL L-1 for 12 hours and then, stored under a modified atmosphere at 25ºC for 23 days. Firmness, total and soluble pectin and cell wall enzymes were monitored during storage. 1-MCP at 300 nL L-1 for 12 hours delayed significantly softening of sapodilla for 11 days at 25ºC. 1-MCP postharvest treatment affected the activities of cell wall degrading enzymes pectinmethylesterase and polygalacturonase and completely suppressed increases in beta-galactosidase for 8 days, resulting in less pectin solubilization. Beta-galactosidase seems relevant to softening of sapodilla and is probably responsible for modification of both pectin and xyloglucan-cellulose microfibril network.; 2008

Qualidade das silagens de três híbridos de sorgo ensilados em cinco diferentes estádios de maturação.

ARAUJO, V. L.; RODRIGUEZ, N. M.; GONCALVES, L. C.; RODRIGUES, J. A. S.; BORGES, I.; BORGES, A. L. C. C.; SALIBA, E. O. S.
Fonte: Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 59, n. 1, p. 168-174, fev. 2007. Publicador: Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 59, n. 1, p. 168-174, fev. 2007.
Tipo: Artigo em periódico indexado (ALICE)
Português
Relevância na Pesquisa
46.015483%
Foram avaliadas as silagens de três híbridos de sorgo de média altura (BR700, BR701 e MASSA 03). Os híbridos foram cortados em cinco diferentes estádios de maturação. Foram avaliados os teores de matéria seca total, pH, teores de nitrogênio amoniacal em porcentagem de nitrogênio total, porcentagem de proteína bruta, frações fibrosas, digestibilidade in vitro da matéria seca e teores de ácidos orgânicos. O delineamento experimental utilizado foi de blocos inteiramente ao acaso com três repetições. Os tratamentos foram arranjados em esquema fatorial 5x3 (estádio de maturação ´ híbrido). As médias foram comparadas pelo teste SNK (P<0,05). Os teores de matéria seca e os valores de pH aumentaram com o avanço do estádio de maturação para os três híbridos estudados. Os valores de pH foram sempre menores que 4,09. Os teores de nitrogênio amoniacal em porcentagem de nitrogênio total, frações fibrosas e digestibilidade in vitro da matéria seca não variaram com o avanço do estádio de maturação. Os valores dos ácidos orgânicos encontrados nas silagens indicaram que o processo fermentativo foi suficiente para boa preservação da forrageira.; 2007

Avaliação pela técnica semiautomática de produção de gases das silagens de quatro genótipos de girassol (Helianthus annus) (Rumbosol 91, Victoria 627, Victoria 807 e Mycogen 9338)

JAYME, D. G.; GONSALVES, L. C.; MAURÍCIO, R. M.; RODRIGUES, J. A. S.; RODRIGUEZ, N. M.; BORGES, A. L. C. C.; BORGES, I.; SALIBA, E. O. S.; PIRES, D. A. A.; GUIMARAES JUNIOR, R.; JAYME, C. G.; PEREIRA, L. G. R.
Fonte: Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 61, n. 6, p. 1403-1410, 2009. Publicador: Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 61, n. 6, p. 1403-1410, 2009.
Tipo: Artigo em periódico indexado (ALICE)
Português
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Foram avaliadas as silagens de quatro genótipos de girassol (Helianthus annus) (Rumbosol 91, Victoria 627, Victoria 807 e Mycogen 93338), pela técnica semiautomática de produção de gases. O delineamento experimental utilizado foi o de blocos ao acaso, em esquema de parcelas subdivididas, sendo as médias comparadas pelo teste SNK (p<0,05). O genótipo Rumbosol 91 apresentou a maior produção acumulativa de gases para o tempo de 96 horas de incubação com valor de 128,47mL/g de MS, e o menor valor foi observado para o genótipo Victoria 807 com 92,88. Não foram observadas diferenças entre os valores de degradabilidade da matéria seca (DMS) para as silagens avaliadas nos diferentes tempos. O potencial máximo de produção de gases variou de 91,67 para o genótipo Victoria 807 a 125,46mL/g de MS para a silagem do genótipo Rumbosol 46,39 e 43,26%. O maior valor para taxa de passagem (8%) foi observado para o genótipo Victoria 627. As silagens dos genótipos Rumbosol 91 e Victoria 627 mostraram destacado potencial para produção de gases e taxa de produção de gases.; 2009

Mapeamento de QTLs de características sob influência da ferrugem asiática da soja.

SANTOS, J. V. M.; PASSIANOTTO, A. L. L.; YAMANAKA, N.; ARIAS, C. A. A.; ABDELNOOR, R. V.
Fonte: In: CONGRESSO BRASILEIRO DE GENÉTICA, 56., 2010, Guarujá. Resumos... [Curitiba]: UFPR, 2010. p. 184. Publicador: In: CONGRESSO BRASILEIRO DE GENÉTICA, 56., 2010, Guarujá. Resumos... [Curitiba]: UFPR, 2010. p. 184.
Tipo: Resumo em anais de congresso (ALICE)
Português
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A ferrugem asiática da soja, causada pelo fungo Phakopsora pachyrhizi (Syd. & P.Syd.), é um dos principais fatores bióticos causadores de prejuízos de redução do potencial sobre a cultura da soja, podendo causar redução de produtividade superiores a 75%. Hoje, sabe-se que uma das formas mais eficazes de controle da doença é através da resistência genética. Devido à baixa durabilidade de genes de resistência vertical, estudos para o desenvolvimento de linhagens com genes de resistência horizontal são de extrema importância. Assim, objetivou-se nesse trabalho identificar QTLs em soja para características agronômicas sob influência da ferrugem asiática. Métodos: 83 marcadores de microssatélites e 2 marcadores morfológicos foram utilizados para a construção de um mapa genético em uma população de linhagens endogâmicas recombinantes (RILs) Ao mesmo tempo, foram feitas avaliações fenotípicas para várias características correlacionadas a doença nas RILs sob ação do patógeno em campo experimental da Embrapa Soja e em fitotron no Japão, a fim de buscar QTLs relacionados à resistência horizontal ao patógeno, e também para verificar a existência de linhagens com maior nível de resistência à doença. Resultados: Uma cobertura parcial de 1.023...

Ensaio de qualidade industrial de trigo - EQIT-2008.

CAMPOS, L. A. C.; BASSOI, M. C.; ALMEIDA, J. L. de; SCHEEREN, P. L.; MARCHIORO, V. S.; ROSA FILHO, O.; MACHADO, J. C.
Fonte: In: REUNIÃO DA COMISSÃO BRASILEIRA DE PESQUISA DE TRIGO E TRITICALE, 3., 2009, Veranópolis. Ata e resumos... Veranópolis: Comissão Brasileira de Pesquisa de Trigo e Triticale: FEPAGRO: ASAV; Passo Fundo: Embrapa Trigo, 2009. 3 p. Subcomissão Melhoramento, trab. 45. 1 CD-ROM. Organizado por Ricardo Lima de Castro, Simone Lancini, André Dabdab Abichequer, Bernadete Radin, Bruno Lisboa, Douglas Lau, Fabiano Paganella, João Leonardo Fernandes Pires, João Rodolfo Guimarães Nunes, Luciano Kayser Vargas, Nelson Gomes Bertoldo. Publicador: In: REUNIÃO DA COMISSÃO BRASILEIRA DE PESQUISA DE TRIGO E TRITICALE, 3., 2009, Veranópolis. Ata e resumos... Veranópolis: Comissão Brasileira de Pesquisa de Trigo e Triticale: FEPAGRO: ASAV; Passo Fundo: Embrapa Trigo, 2009. 3 p. Subcomissão Melhoramento, trab. 45. 1 CD-ROM. Organizado por Ricardo Lima de Castro, Simone Lancini, André Dabdab Abichequer, Bernadete Radin, Bruno Lisboa, Douglas Lau, Fabiano Paganella, João Leonardo Fernandes Pires, João Rodolfo Guimarães Nunes, Luciano Kayser Vargas, Nelson Gomes Bertoldo.
Tipo: Artigo em anais de congresso (ALICE)
Português
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2009

Uso de planejamento experimental na produção de lipase fúngica a partir de farelo de trigo.

SANTOS, R. R. S.; MURUCI, L. N. M.; DAMASO, M. C. T.; SANTOS, L. O; SILVA, J. P. L
Fonte: In: SEMINÁRIO BRASILEIRO DE TECNOLOGIA ENZIMÁTICA - ENZITEC, 10., Blumenau, SC. [Anais...]. Blumenau: Fundação Universidade Regional de Blumenau, 2012. Publicador: In: SEMINÁRIO BRASILEIRO DE TECNOLOGIA ENZIMÁTICA - ENZITEC, 10., Blumenau, SC. [Anais...]. Blumenau: Fundação Universidade Regional de Blumenau, 2012.
Tipo: Resumo em anais de congresso (ALICE) Formato: Não paginado.
Português
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2012

A network landscape from CNVs to Nellore cattle beef tenderness.

SILVA, V. H.; GIACHETTO, P. F.; TIZIOTO, P. L.; GONÇALVES, T. M.; REGITANO, L. C. A.; COUTINHO, L. L.
Fonte: In: INTERNATIONAL SYMPOSIUM ON ANIMAL FUNCTIONAL GENOMICS, 5., 2013, Guarujá. Programme and abstract book... [S.l.: s.n.], 2013. Publicador: In: INTERNATIONAL SYMPOSIUM ON ANIMAL FUNCTIONAL GENOMICS, 5., 2013, Guarujá. Programme and abstract book... [S.l.: s.n.], 2013.
Tipo: Resumo em anais de congresso (ALICE) Formato: p. 8.
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This work aims to perform a couple of in silico network analysis from CNVs standpoint, shedding light to possible metabolic connections to tenderness. It was used 671 Nellore males to infer CNVs, through SNP-chip (Illumina Bovine HD Beadchip®, containing approximately 770 thousand SNPs) and PennCNV software methodology. CNV regions (CNVRs) were inferred by CNVRuler (recurrence 0.1).; 2013; ISAFG 2013. AB.01.

Contamina????o qu??mica e microbiol??gica na cadeia produtiva do p??ssego: contribui????o ?? an??lise de risco; Contamina????o qu??mica e microbiol??gica na cadeia produtiva do p??ssego: contribui????o ?? an??lise de risco

SCHNEIDER, Evandro Pedro
Fonte: Universidade Federal de Pelotas; Agronomia; Programa de P??s-Gradua????o em Agronomia; UFPel; BR Publicador: Universidade Federal de Pelotas; Agronomia; Programa de P??s-Gradua????o em Agronomia; UFPel; BR
Tipo: Tese de Doutorado Formato: application/pdf
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Identifying contamination potential risk in the peach production system is necessary to the construction of critical consciousness about the topic, as well as to the improvement of problem control in the productive chain such as Integrated Production of Fruits. From this issue, it is proposed to identify and supervise the farmers profile, their current level of contamination and monitor the peach chain, related to the presence of pesticides residues and microbiological contamination, in order to identify the vulnerabilities and risk situations connected to the use of pesticides and microbiological contamination in the peach productive chain, in the region of Pelotas, RS. As work methodology it was applied questionnaires, blood collected from producers (biological analyze), sample of fruits and water were collected during two consecutives harvests (2010/11 and 2011/12) in 25 productive farms. According to the diagnosis made, the lower education, the use of badly calibrated equipments, added to the insufficient technical assistance, constitute vulnerabilities related to the inadequate use of pesticides. The water used in the rural properties presents not only microbiological but also pesticide contamination. According to the percentages of unacomplishment of Maximum Limits of Residues and the inadequate use of pesticides non-authorized to the culture...

Resposta de capim p??-de-galinha (Eleusine spp.) ao herbicida glyphosate; Response of goosegrass (Eleusine spp.) to the herbicide glyphosate

ULGUIM, Andr?? da Rosa
Fonte: Universidade Federal de Pelotas; Agronomia; Programa de P??s-Gradua????o em Fitossanidade; UFPel; BR Publicador: Universidade Federal de Pelotas; Agronomia; Programa de P??s-Gradua????o em Fitossanidade; UFPel; BR
Tipo: Dissertação Formato: application/pdf
Português
Relevância na Pesquisa
46.003066%
The goosegrass (Eleusine spp.) is an annual plant, found in agricultural crops in Rio Grande do Sul (RS), where has been control failures in response to glyphosate application, suggesting the resistance of the species to this herbicide. The objectives of this research were to identify the occurrence of resistance of goosegrass to glyphosate in RS, determine the LD50 and GR50, identify the mechanism of resistance, evaluate the factors involved in the observed control failures, and propose alternatives to chemical control to these biotypes. Thus, plant seeds of failures herbicide control, suspected of resistance, were collected in eight cyties soybean producers in RS, and used in the experiments. The results showed that all the plants from seeds collected has been controlled by glyphosate when applied at a rate of 2160 g a.e. ha-1. The biotype 12.3, from Boa Vista do Incra, show a low level resistance to glyphosate, with resistance factor (RF) of 1.17, according to LD50, and the mechanism that confers resistance is not due to the metabolism of glyphosate by cyt-P450 complex, or herbicide absorption. The suspected resistant biotypes have lower control when glyphosate is sprayed in advanced stages of growth, as one or two tillers, that may be the cause of control failures observed in crop area. The ACCase inhibiting herbicides (clethodim and fluazifop-p-butyl) and amonium-glufosinate efficiently control these biotypes...

Caracterizaci??n de las enzimas esterasas implicadas en la interacci??n polen-pistilo en el olivo (Olea Europaea L.)

Rej??n Garc??a, Juan David
Fonte: Universidad de Granada Publicador: Universidad de Granada
Tipo: Tese de Doutorado
Português
Relevância na Pesquisa
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El ??xito de la fertilizaci??n en las Angiospermas depende en gran medida de la capacidad del polen para germinar y emitir un tubo pol??nico, el cu??l transporta los n??cleos esperm??ticos a trav??s del pistilo hasta el saco embrionario. En este contexto, los objetivos de este proyecto fueron: 1) determinar la existencia de actividad esterol??tica en el polen de olivo, 2) identificar y clasificar desde el punto de vista funcional las distintas enzimas esterasas, 3) determinar las funciones del exudado estigm??tico y el 'pollen coat', y 4) caracterizar a nivel bioqu??mico y molecular la enzima pectina metilesterasa del polen. El polen de olivo contiene al menos 14 enzimas esterasas, agrupadas en tres clases: carboxilesterasas (2), acetilesterasas (6) y colinesterasas (6). An??lisis funcionales mediante inhibidores qu??micos espec??ficos mostraron un efecto adverso sobre la capacidad de germinar del polen y, en el caso del inhibidor DIFP, una disminuci??n significativa de la longitud del tubo pol??nico. El polen de olivo tambi??n posee al menos 12 enzimas esterasa/lipasa capaces de hidrolizar ??steres de cadena larga. Las distintas enzimas presentaron una localizaci??n celular espec??fica en el grano de polen y en el tubo pol??nico. Los datos obtenidos sugieren que las enzimas esterasas del polen est??n implicadas en la modificaci??n selectiva de la pared celular del tubo pol??nico...