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Higiene e Desenvolvimento Urbano: O Porto na transição entre os séculos XIX e XX

Stefanie Braz André
Fonte: Universidade do Porto Publicador: Universidade do Porto
Tipo: Dissertação
Português
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This thesis aims to evidence the importance of hygiene in urban development, and also tohighlight the repercussions that arose in regard to architectural works, mainly those of a publicnature.An analysis of a specific time period was conducted throughout this essay, originally focusingon the study of an industrialised city, while mentioning its effects on the lives of people, thecitys urban fabric and its resulting consequences.Furthermore, the city of Paris was used as a reference for a comparative study with the city ofPorto, as well as theories of other pioneers of the quot;underground urbanismquot; (e.g. Pierre Patte,Georges-Eugène Haussmann, Eugène Belgrand, Eugène Hénard, et al). In addition, both citiestopographical issues were equally taken into consideration.To understand better the city of Porto during its transition between the second half of thenineteenth century and the first half of the twentieth century, an analysis was performed of theperiods social and political matters, including the outcome of sanitary evolution and theconstraints imposed on improving hygiene.It is made known through Ricardo Jorges work Portos real panorama, regarding its last bubonicplague incident and the fundamental keys to good public health.The discovery of bacteriology provided another insight into water quality...

Undergraduation students attitudes toward the use of concept maps in a bacteriology curricular unit

António Pedro Fonseca; Carlinda Leite; Clara Isabel Extremina; Ian Kinchin; Luísa Peixe
Fonte: Universidade do Porto Publicador: Universidade do Porto
Tipo: Artigo de Revista Científica
Português
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Characterization of the yeast population from tradiotional corn and rye bread doughs

Almeida, M. J.; Pais, Célia
Fonte: The Society for Applied Bacteriology Publicador: The Society for Applied Bacteriology
Tipo: Artigo de Revista Científica
Publicado em //1996 Português
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Yeasts were isolated from a variety of home-made bread doughs and identified. A pure culture of Saccharomyces cerevisiae was found in 18%of the doughs. The same species predominated in 80% of the doughs examined whereas Issatchenkia orientalis, Pichia membranaefaciens and Torulaspora delbrueckii were present in about 40% of the samples. About one quarter of the isolates displayed killer activity, strains of P. anomala showing the broadest spectra. Two isolates of S. cerevisiae and three of T. delbrueckii gave biomass values in sucrose medium similar to or higher than those obtained with commercial compressed baker´s yeast strains.

Development of an optimized technique for the recovery of H. pylori from water and drinking water biofilms

Azevedo, N. F.; Vieira, M. J.; Keevil, C. W.
Fonte: Universidade do Minho Publicador: Universidade do Minho
Tipo: Parte de Livro
Publicado em /09/2005 Português
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At present, the route of transmission is perhaps one of the most controversialareas of Helicobacter pylori research. Drinking water and associated biofilms have been suggested as possible environmental reservoirs for the bacterium, however, successful and consistent detection of the bacterium in real systems has not yet been accomplished. A new protocol that offers improved recovery ratings of H. pylori from water and associated-biofilms has been developed and evaluated. This protocol includes optimized incubation atmospheres and a new recovery culture medium, as well as an assessmentof existing techniques for the detachment of H. pylori from surfaces. Even though obvious advantages in terms of cell counts are obtained using this method, certain problems, such as contamination by faster-growing species of the culture medium in heavily contaminated waters may still hinder a successful recovery of the pathogen.Future research will involve further improvement of the protocol and monitoring of real drinking water distribution systems.; Unit of Helicobacter/Campylobacter of the Center of Bacteriology of INSA para a Ciência e a Tecnologia (FCT) ; European Commission Research Project SAFER.

Influence of anaerobic conditions on vaginal microbiota recovery from bacterial vaginosis patients

Cereija, Tatiana; Castro, J.; Alves, P.; Cerca, Nuno
Fonte: BMJ Publishing Group; BMJ Publishing Group Publicador: BMJ Publishing Group; BMJ Publishing Group
Tipo: Artigo de Revista Científica
Publicado em //2013 Português
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Bacterial vaginosis (BV) is one of the most common infections in women of reproductive age. Clinical studies have shown an association among BV and abnormal pregnancy, pelvic inflammatory disease and increased risk of sexually transmitted infections, including HIV. This disorder was first described in 1914 by Curtis as a “white discharge” syndrome and despite the decades of research we have only limited, and clearly not conclusive, evidence of microbial cause of BV, mechanism of disease and effective treatment. The development of molecular techniques such as Denaturing Gradient Gel Electrophoresis (DGGE) and DNA sequencing produced a clearer picture of the complexities of the vaginal microbiota. It has also become more apparent that none of the microorganisms already isolated from the vagina are likely to be the sole pathogen responsible for BV. Thus, improved knowledge of the relationship between different species of bacteria and their host is critical to a better understanding of both women’s health and illness. In order to comprehend the dynamic interaction between bacteria–bacteria and bacteria–host it becomes necessary to isolate bacteria from vaginal samples and to use them in in vitro and in vivo assays. The human vagina has a very specific environment regarding pH...

Vigilância epidemiológica da infecção do local cirúrgico nas artroplastias da anca e do joelho

Maia, Maria José dos Santos
Fonte: Universidade Católica Portuguesa Publicador: Universidade Católica Portuguesa
Tipo: Dissertação de Mestrado
Publicado em 23/12/2011 Português
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Introdução - A Vigilância Epidemiológica (VE) é a área nobre da Prevenção e Controlo da Infecção, sendo “… fundamental assegurar o registo contínuo, a análise, a interpretação e a informação de retorno relativamente às Infecções Associadas aos Cuidados de Saúde (IACS) …”. (1) A revisão da literatura relacionada com o tema, incidiu sobre as IACS, o sistema de VE proposto pela Direcção Geral da Saúde (DGS), a Infecção do Local Cirúrgico (ILC), as Artroplastias da Anca e do Joelho e sobre as Recomendações existentes para a Prevenção e Controlo da ILC, tanto a nível nacional como a nível internacional. Objectivo - Conhecer as ILC nas Artroplastias da Anca e do Joelho num Hospital Central. Metodologia - Efectuou-se um estudo do tipo descritivo, prospectivo e de abordagem quantitativa. Avaliaram-se 626 procedimentos cirúrgicos, de acordo com o sistema de codificação – ICD-9-CM. Foram eles a Substituição Total da Anca (STA) - 81.51, a Substituição Parcial da Anca (SPA) - 81.52, a Revisão da Substituição da Anca (RSA) - 81.53, a Substituição Total do Joelho (STJ) - 81.54 e a Revisão da Substituição do Joelho (RSJ) - 81.55. Excluíram-se 27 procedimentos cirúrgicos (81.53 e 81.55) relativos a 18 doentes...

A imunodepressão como factor de risco na colonização bacteriana de cateteres intravenosos em animais de companhia hospitalizados

Pires, Lara Sofia Fernandes Ribeiro da Silva
Fonte: Universidade Técnica de Lisboa. Faculdade de Medicina Veterinária Publicador: Universidade Técnica de Lisboa. Faculdade de Medicina Veterinária
Tipo: Trabalho de Conclusão de Curso
Publicado em //2008 Português
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Dissertação de Mestrado Integrado em Medicina Veterinária; A colonização bacteriana de cateteres intravenosos (CBCI) pode conduzir a infecções nosocomiais e a septicemia em animais em estado crítico. A imunossupressão do hospedeiro pode predispor para a CBCI. Este estudo propõe a comparação entre a prevalência de CBCI em animais hospitalizados com condições de imunodepressão e em animais sem esta condição. Também se pretende investigar o papel de outros factores de risco conhecidos para a CBCI. População em estudo: animais internados no hospital escolar da FMV – UTL, entre Novembro de 2007 e Março de 2008. Critérios de inclusão: cateteres colocados por um período mínimo de 6 horas, após tricotomia e preparação asséptica do local de cateterização. Foram criados dois grupos em função da presença ou da ausência de condição imunodepressora, (grupos I e NI, respectivamente). Processamento dos cateteres: Os cateteres foram removidos em condições de assepsia. As cânulas foram cultivadas segundo técnicas de bacteriologia qualitativa. Variáveis estudadas: diâmetro do cateter, idade do paciente, género e espécie, tipo de condição imunodepressora, duração de cateterização e utilização de antibióticos. Análise de dados: PROC GENMON e PROC GLM...

Bacteriologia ocular em canídeos: estudo retrospectivo 2002-2010

Subtil, Sandra Daniela de Oliveira
Fonte: Universidade Técnica de Lisboa. Faculdade de Medicina Veterinária Publicador: Universidade Técnica de Lisboa. Faculdade de Medicina Veterinária
Tipo: Trabalho de Conclusão de Curso
Publicado em 02/11/2010 Português
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Dissertação de Mestrado Integrado em Medicina Veterinária; O objectivo deste estudo foi avaliar a microbiota ocular presente em cães com sinais de doença ocular externa, bem como o seu padrão de susceptibilidade a agentes antimicrobianos. Os isolados bacterianos, num total de 91, foram obtidos a partir de zaragatoas oculares colhidas de 76 canídeos com sinais de doença ocular externa. As amostras foram obtidas entre Janeiro de 2002 e Março de 2010, sendo processadas no Laboratório de Bacteriologia da FMV/UTL. Os testes de susceptibilidade a agentes antimicrobianos foram realizados pelo método de difusão em disco (NCCLS, 2002; CLSI, 2008). As bactérias isoladas foram sobretudo Gram-positivas (76,9%), o género predominante foi Staphylococcus sp. (45%) e as espécies mais frequentes foram Staphylococcus aureus, Staphylococcus pseudintermedius e Streptococcus canis, numa percentagem equitativa de 13,2%. Das bactérias Gram-negativas o género mais frequentemente isolado foi Pseudomonas sp. (9,9%) e, dentro deste, a espécie Pseudomonas aeruginosa (6,6%). Foi observada resistência a pelo menos um fármaco em 93,5% dos antibiogramas. Todos os staphylococci coagulase positivos testados exibiram resistências aos princípios activos amicacina...

Estudo longitudinal da eficácia da enrofloxacina no tratamento da infecção do tracto urinário complicada no cão

Dias, Inês Freire Norberto
Fonte: Universidade Técnica de Lisboa. Faculdade de Medicina Veterinária Publicador: Universidade Técnica de Lisboa. Faculdade de Medicina Veterinária
Tipo: Dissertação de Mestrado
Publicado em 21/11/2011 Português
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Dissertação de Mestrado Integrado em Medicina Veterinária; As infecções do tracto urinário complicadas estão associadas a alterações estruturais ou funcionais do tracto genitourinário ou à presença de doenças concomitantes que interfiram com os mecanismos de defesa do hospedeiro o que aumenta o risco de adquirir infecção ou falha terapêutica. O objectivo deste estudo foi avaliar as propriedades bactericida e concentração - dependente da enrofloxacina quando utilizada no dobro da dose padrão, uma vez ao dia durante 7 dias no tratamento da ITUc no cão. Durante 4 meses, 14 cães com sinais de ITU inferior, factores que predispusessem a uma potencial ITUc e sem antibioterapia prévia foram incluídos neste estudo. Os pacientes foram avaliados clinicamente (através de um inquérito) e a urina foi colhida para urianálise e urocultura aos dias 0, 3, 7 e 12. A urocultura e os testes de susceptibilidade a antibióticos foram realizados segundo os métodos padrão e interpretados segundo os critérios do CLSI. Dos 14 pacientes, 7 apresentaram bacteriúria significativa (BS) por Escherichia coli (n=3), Staphylococcus cohnii (n=1), Proteus mirabilis (n=1), Enterococcus faecalis (n=1), e um Staphylococcus aureus meticilina - resistente (MRSA) caracterizados por: >105 UFC/ml...

A lavagem traqueal e a lavagem broncoalveolar como métodos de diagnóstico da doença respiratória em equinos

Silva, Nair Almeida
Fonte: Universidade Técnica de Lisboa. Faculdade de Medicina Veterinária Publicador: Universidade Técnica de Lisboa. Faculdade de Medicina Veterinária
Tipo: Dissertação de Mestrado
Publicado em 02/05/2012 Português
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Dissertação de Mestrado Integrado em Medicina Veterinária; A doença respiratória é uma das causas mais importantes da má performance atlética em cavalos de desporto em todo o mundo. Actualmente estão disponíveis um grande número de ferramentas e exames complementares que permitem uma avaliação minuciosa do aparelho respiratório. É importante para os veterinários conhecer as principais indicações, as informações passíveis de ser obtidas através do uso correcto destes meios complementares para se chegar ao diagnóstico definitivo específico. O objectivo geral deste trabalho consistiu na implementação e desenvolvimento de um protocolo para a colheita, processamento e avaliação das secreções respiratórias em equinos adequado para o funcionamento do hospital veterinário onde este trabalho foi desenvolvido. Secundariamente pretendeu-se verificar os tipos celulares obtidos na lavagem traqueal e na lavagem broncoalveolar em equinos sem história clínica e sinais de doença respiratória e, deste modo inferir sob a importância e aplicabilidade de ambas as técnicas no estudo da patologia respiratória. Com este trabalho constatámos a predominância das células globulares, células colunares ciliadas e de macrófagos alveolares na lavagem traqueal. Dois dos cinco equinos seleccionados para colheita apresentaram hiperplasia linfóide folicular diagnosticada através da endoscopia não tendo demonstrado nenhuma sintomatologia no exame físico salientando a importância da conjugação de todas os dados recolhidos durante todo o processo de diagnóstico.; ABSTRACT - The tracheal wash and bronchoaveolar lavage as methods used in the diagnosis of equine respiratory disease - The respiratory disease is one of the most important cause of poor athletic performance in race horses worldwide. Nowadays there is a large number of tools and ancillary diagnostic techniques that can be used to perform an accurate examination of the upper and lower respiratory tract. It is important for all the veterinarians to know the major indications...

A multitask ATPase serving different ABC-type sugar importers in Bacillus subtilis

Sá-Nogueira, Isabel de; Ferreira, Mário José
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em //2010 Português
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Journal of Bacteriology (Out 2010) 5312-5318; Bacillus subtilis is able to utilize arabinopolysaccharides derived from plant biomass. Here, by combining genetic and physiological analyses we characterize the AraNPQ importer and identify primary and secondary transporters of B. subtilis involved in the uptake of arabinosaccharides. We show that the ABC-type importer AraNPQ is involved in the uptake of α-1,5-arabinooligosaccharides, at least up to four L-arabinosyl units. Although this system is the key transporter for α-1,5-arabinotriose and α-1,5-arabinotetraose, the results indicate that α-1,5-arabinobiose also is translocated by the secondary transporter AraE. This broad-specificity proton symporter is the major transporter for arabinose and also is accountable for the uptake of xylose and galactose. In addition, MsmX is shown to be the ATPase that energizes the incomplete AraNPQ importer. Furthermore, the results suggest the existence of at least one more unidentified MsmX-dependent ABC importer responsible for the uptake of nonlinear α-1,2- and α-1,3-arabinooligosaccharides. This study assigns MsmX as a multipurpose B. subtilis ATPase required to energize different saccharide transporters, the arabinooligosaccharide-specific AraNPQ-MsmX system...

Characterization of the abn2(yxiA) encoding a Bacillus subtilis GH43 arabinanase, Abn2, and its role in arabino-polysaccharides degradation.

Sá-Nogueira, Isabel de; Inácio, José Manuel
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /04/2008 Português
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Journal of Bacteriology (Junho 2008) 4272-4280; The extracellular depolymerization of arabinopolysaccharides by microorganisms is accomplished by arabinanases, xylanases, and galactanases. Here, we characterize a novel endo-alpha-1,5-l-arabinanase (EC 3.2.1.99) from Bacillus subtilis, encoded by the yxiA gene (herein renamed abn2) that contributes to arabinan degradation. Functional studies by mutational analysis showed that Abn2, together with previously characterized AbnA, is responsible for the majority of the extracellular arabinan activity in B. subtilis. Abn2 was overproduced in Escherichia coli, purified from the periplasmic fraction, and characterized with respect to substrate specificity and biochemical and physical properties. With linear-alpha-1,5-l-arabinan as the preferred substrate, the enzyme exhibited an apparent K(m) of 2.0 mg ml(-1) and V(max) of 0.25 mmol min(-1) mg(-1) at pH 7.0 and 50 degrees C. RNA studies revealed the monocistronic nature of abn2. Two potential transcriptional start sites were identified by primer extension analysis, and both a sigma(A)-dependent and a sigma(H)-dependent promoter were located. Transcriptional fusion studies revealed that the expression of abn2 is stimulated by arabinan and pectin and repressed by glucose; however...

trans-Acting factors and cis elements involved in glucose repression of arabinan degradation in Bacillus subtilis.

Sá-Nogueira, Isabel de; Inácio, José Manuel
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /11/2007 Português
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Journal of Bacteriology (Nov 2007) 8371-8376; In Bacillus subtilis, the synthesis of enzymes involved in the degradation of arabinose-containing polysaccharides is subject to carbon catabolite repression (CCR). Here we show that CcpA is the major regulator of repression of the arabinases genes in the presence of glucose. CcpA acts via binding to one cre each in the promoter regions of the abnA and xsa genes and to two cres in the araABDLMNPQ-abfA operon. The contributions of the coeffectors HPr and Crh to CCR differ according to growth phase. HPr dependency occurs during both exponential growth and the transitional phase, while Crh dependency is detected mainly at the transitional phase. Our results suggest that Crh synthesis may increase at the end of exponential growth and consequently contribute to this effect, together with other factors.

Functional domains of Bacillus subtilis transcription factor AraR and identification of aminoacids important for nucleoprotein complex assembly and effector-binding.

Sá-Nogueira, Isabel de; Franco, Irina Saraiva; Mota, Luís Jaime; Soares, Cláudio Manuel
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /03/2006 Português
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Journal of Bacteriology (Apr 2006) 3024-3036; The Bacillus subtilis AraR transcription factor represses at least 13 genes required for the extracellular degradation of arabinose-containing polysaccharides, transport of arabinose, arabinose oligomers, xylose, and galactose, intracellular degradation of arabinose oligomers, and further catabolism of this sugar. AraR exhibits a chimeric organization comprising a small N-terminal DNA-binding domain that contains a winged helix-turn-helix motif similar to that seen with the GntR family and a larger C-terminal domain homologous to that of the LacI/GalR family. Here, a model for AraR was derived based on the known crystal structures of the FadR and PurR regulators from Escherichia coli. We have used random mutagenesis, deletion, and construction of chimeric LexA-AraR fusion proteins to map the functional domains of AraR required for DNA binding, dimerization, and effector binding. Moreover, predictions for the functional role of specific residues were tested by site-directed mutagenesis. In vivo analysis identified particular amino acids required for dimer assembly, formation of the nucleoprotein complex, and composition of the sugar-binding cleft. This work presents a structural framework for the function of AraR and provides insight into the mechanistic mode of action of this modular repressor.

Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by desulfovibrio alaskensis NCIMB 13491

Mota, Cristiano S.; Valette, Odile; J. González, Pablo; Brondino, Carlos D.; Moura, José J. G.; Moura, Isabel; Dolla, Alain; Rivas, Maria G.
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /04/2011 Português
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Journal of Bacteriology. 2011 Jun; Vol. 193 issue 12 pages 2917-2923; Formate dehydrogenases (FDHs) are enzymes that catalyze the formate oxidation to carbon dioxide and that contain either Mo or W in a mononuclear form in the active site. In the present work, the influence of Mo and W salts on the production of FDH by Desulfovibrio alaskensis NCIMB 13491 was studied. Two different FDHs, one containing W (W-FDH) and a second incorporating either Mo or W (Mo/W-FDH), were purified. Both enzymes were isolated from cells grown in a medium supplemented with 1 M molybdate, whereas only the W-FDH was purified from cells cultured in medium supplemented with 10 M tungstate. We demonstrated that the genes encoding the Mo/W-FDH are strongly downregulated by W and slightly upregulated by Mo. Metal effects on the expression level of the genes encoding the W-FDH were less significant. Furthermore, the expression levels of the genes encoding proteins involved in molybdate and tungstate transport are downregulated under the experimental conditions evaluated in this work. The molecular and biochemical properties of these enzymes and the selective incorporation of either Mo or W are discussed.

Efecto de la temperatura de la piel en la leishmaniasis cutanea experimental

Zeledón,Rodrigo
Fonte: Sociedade Brasileira de Medicina Tropical - SBMT Publicador: Sociedade Brasileira de Medicina Tropical - SBMT
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/1971 Português
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The literature on the thermosensitive properties of strains or species of Leishmania and of other miercorganisms is revised. Cutaneous or mucocutaneous strains that infect animais in the coldest areas of the skin or mucosa in general can not grow in tissue culture at 37°C or higher temperatures and their respiratory metabolism decreases at these temperatures. These facts suggest a thermosensitive event in some important metabolism phase of the organisme. The strains or species that are able to produce visceral leishmaniasis were probably originated from cutaneous strains after genetioally determined physiological adaptation, to warmer temperatures. These strains can not only visceralize in animais and man but will also grow in tissue culture at 36-37°C and the respiratory metabolism will be higher at such temperatures. There are reasons to believe that intermediate strains, i. e., with properties of both groupsí do exist. A thermosensitive physiological event is a more general phenomenon and examples of it can also be found in the fields of virology, bacteriology and mycology. It has practical applications since some of the diseases produced by these agents can be cured by treatments with heat or artificial fever. Experiments along these line were performed on hamsters with a Costa Rican strain of L. braziliensis as an experimental model. Even after intraperitoneal inoculation lesions appear in the nose...

Endocardites sépticas: (contribuição etiopatogênica)

Lacorte,J. Guilherme; Santos,Mario
Fonte: Instituto Oswaldo Cruz, Ministério da Saúde Publicador: Instituto Oswaldo Cruz, Ministério da Saúde
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/08/1944 Português
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In the present paper the A. A. reviewed the more fundamental problems in the ethiopathogenic process of bacterial endocardits and report the bacte¬riological observations done by them in 20 cases of the disecase observed at the Section of Pathological Anatomy and Bacteriology of the Hospital S. Francisco de Assis in charge of the Instituto Oswaldo Cruz. The A.A. isolated Pneumococcus from 10 out the 20 cases, Gonococcus from 2, Staphylococcus from 2, Streptococcus from 5 and Friedlaender bacil¬lus from 1 . The A.A. mainly lay stress on the necessity of the bacteriological exa¬minations being made by a specialist, owing to the difficulties sometimes met with and to the consequences of a detailed examination of the isolated germ.

Assessing the histopathology to depict the different stages of bovine tuberculosis infection in a naturally infected herd

Medeiros,Luciana S.; Marassi,Carla D.; Figueiredo,Eduardo E.S; Leite,Juliana; Ferreira,Ana Maria R.; Lilenbaum,Walter
Fonte: Colégio Brasileiro de Patologia Animal - CBPA; Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Publicador: Colégio Brasileiro de Patologia Animal - CBPA; Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/02/2012 Português
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The standard method for detection of bovine tuberculosis (TB) is the single intradermal tuberculin test (SITT). Nevertheless, current studies suggest that a single test is not enough to detect all cattle infected by TB, particularly when animals present different stages of infection. A dairy herd comprised of 270 cows was studied and 15 were reactive to SITT plus nine inconclusive animals. Blood samples (for IFN and ELISA) were collected from these 24 cows. At 30 days after injection of PPD, all the cows that were reactive to any of the employed tests were slaughtered, and tissues were processed by Bacteriology, Histopathology (HP) and PCR. According to HP 33.4% of the animals were positive, 45.8% inconclusive and 20.8% were negative. The inconclusive samples came from IFN positive animals, signalizing recent infection. Regarding the animals that were negative to HP, all of them were identified by IFN while ELISA was negative. Immune responses are different in recent and advanced infections, what supports the identification between chronically or recently infected animals. This multidisciplinary approach is mandatory for the interpretation of the various tools that are frequently employed for the diagnosis of TB and mainly to identify all infected animals.

Inconsistency of the biological control of Meloidogyne incognita race 2 in melon by endophytic bacteria

Medeiros,Jeane E de; Mariano,Rosa de LR; Pedrosa,Elvira MR; Silveira,Elineide B da
Fonte: Associação Brasileira de Horticultura Publicador: Associação Brasileira de Horticultura
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/09/2009 Português
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We obtained 61 rhizobacterium isolates from rhizosphere soil samples collected in melon commercial fields located in Mossoró, Rio Grande do Norte State, Brazil. These isolates, along with 56 endophytic bacteria from the Collection of Cultures of the Plant Bacteriology Laboratory of the Universidade Federal Rural de Pernambuco, were tested for controlling Meloidogyne incognita race 2 in melon. To infest the soil with nematodes, 1000 eggs of Meloidogyne incognita race 2 per plant were placed in pots where seedlings of the yellow-type melon, cultivar AF 682, were growing for 10 days. Two days before, 20 mL of bacterial suspension (0.7 OD570nm) were poured into each pot. After 60 days, fresh root biomass, gall index, egg mass, and the nematode reproduction factor were assessed. Among the 117 isolates screened, the endophytic Bacillus ENM7, ENM10, and ENM51 were selected because they significantly reduced egg mass and/or gall index. However, when tested again, separately and in mixtures, these isolates nor confirmed their efficiency in vivo, neither affected juvenile emergence in vitro. These results give evidence on the inconsistency of using endophytic-bacteria in the control of M. incognita race 2 in melon.

Growth and nodulation in soybean cultivated on two substrata and treated with five rates of metolachlor

Amarante,Cassandro Vidal Talamini do; Canci,Paulo Cesar
Fonte: Universidade Federal de Santa Maria Publicador: Universidade Federal de Santa Maria
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/1995 Português
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The effect of metolachlior [2-chloro-N-(2-ethyl-6-methylphenyl)-N-(2-methoxy-l-methylethyl) acetamide] at rates of 0.0, 1.5, 3.0, 4.5, and 6.0kg a.i./ha on the growth and nodulation of soybean cv. BR-4 cultivated on two substrata (soil and soil + sand) and inoculated with Bradyrhizobium japonicum (Kirchnea-1896) Jordan 1982, 137 (Denotes that this name has been validly published in the oficial publicadon, Intemational Joumal of Systematic Bacteriology), was studied under greenhouse conditions at Lages, SC, Brazil, in 1992-93. The experimental design was completely randomized with four replicates. The experimental unit was a container with two plants. Fifty-two days after the emergence (at R1 stage - beginning of flowering) the plants were harvested and evaluated for shoot, root, leaf and nodule dry matter, nodule number, and leaf area. The data were submitted to variance analysis and multiple polynomial regression. Increasing rates of metolachlor significantly reduced shoot, leaf and nodule dry matter and leaf area, the data following a linear polynomial regression, while root dry matter and nodule number were not affected. The interaction "rate x substratum" was not significant for all parameters studied. Except for root dry matter and nodule number...