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Cristalização e análise cristalográfica preliminar da N-acetilglicosamina 6-fosfato desacetilase de Escherichia coli; Crystallization and preliminary crystallographic analysis of the N-aceylglucosamine 6-phosphate deacetylase from Escherichia coli

Ferreira, Frederico Moraes
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 23/05/2000 Português
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A N-acetilglicosamina 6-fosfato desacetilase (EC 3.5.1.25), uma enzima envolvida na via catabólica de açucares aminados da Escherichia coli, foi cristalizada pela técnica de difusão de vapor utilizando-se fosfato como agente precipitante. Experimentos de difração de raios-X mostraram que os cristais pertencem ao sistema cristalino ortorrômbico com grupo espacial P21212. Os parâmetros de cela são a=82,09(2) Å, b=114,50(1) Å e c=80,17(1) Å. Os cristais difrataram a uma resolução máxima de 1,8 Å e um conjunto de dados foi coletado até 2.0 Å. O conteúdo da unidade assimétrica provavelmente é um dímero, produzindo um coeficiente de Matthews de 2,30 Å3 Da-1; N-acetylglucosamine 6-phosphate deacetylase (EC 3.5.1.25), an enzyme involved in amino sugar catabolismo from Escherichia coli has been crystallized by the vapor diffusion technique using phosphate as precipitant. X-ray diffraction experiments show the crystals to belong to the orthorhombic crystals system with space group P21212. The unit cell parameters are a=82,09(2) Å, b=114,50(1) Å e c=80,17(1) Å. The crystals diffract to a maximum resolution of 1.8 Å and a initial dataset was collected to 2.0 Å. The asymmetric unit content is likely to be a dimer...

Leveduras de cerveja e cana-de-açúcar (Saccharomyces cerevisiae), autolisada e íntegra, na dieta de cães

Martins, Mariana dos Santos
Fonte: Universidade Estadual Paulista (UNESP) Publicador: Universidade Estadual Paulista (UNESP)
Tipo: Dissertação de Mestrado Formato: xiii, 80 f. : il., grafs.
Português
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Pós-graduação em Zootecnia - FCAV; O objetivo do presente estudo foi determinar por meio dos métodos de substituição, os coeficientes de digestibilidade aparente (CDA) das leveduras de cerveja e de cana-de-açúcar autolisada e íntegra, e de inclusão de teores crescentes das leveduras de cana-de-açúcar em dietas extrusadas para cães adultos. Dois ensaios foram conduzidos; no primeiro foi utilizado o método de substituição e 4 dietas experimentais, uma dieta referência isenta de levedura e outras três compostas pela substituição de 15% da dieta basal por leveduras de cerveja e de cana-de-açúcar autolisada e íntegra, respectivamente. Para o segundo ensaio foram utilizados 35 cães adultos distribuídos em 5 tratamentos e 7 repetições por dieta, estas sendo: 0 (basal), 7,5 e 15% de levedura de cana-de-açúcar autolisada e 7,5 e 15% de íntegra. No primeiro ensaio, os CDA da PB são 96,47, 62,98 e 74,70% e da EB 84,01, 68,43 e 75,95%, respectivos as leveduras; havendo diferença entre as leveduras avaliadas apenas para o CDA da PB (p<0,05). No segundo ensaio, os tratamentos não se mostraram diferentes quanto a digestibilidade para os nutrientes MS...

Modificações faciais em clientes submetidos a tratamento estético fonoaudiológico da face em Clínica-Escola de Fonoaudiologia

Arizola,Hilda Gabriela Arantes de; Brescovici,Silvana Maria; Delgado,Susana Elena; Ruschel,Caroline Kurtz
Fonte: CEFAC Saúde e Educação Publicador: CEFAC Saúde e Educação
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2012 Português
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OBJETIVO: identificar possíveis modificações faciais em pacientes submetidos à tratamento estético fonoaudiológico da face na Clínica-Escola de Fonoaudiologia e verificar se estas modificações foram percebidas pelo cliente, por terceiros e por fonoaudiólogos, e constatar o grau de satisfação do cliente com o resultado. MÉTODO: participaram do estudo 11 mulheres com idade de 40 a 50 anos (média de idade 44,5 ± 3,6 anos), excluíram-se as com tratamento fonoaudiológico estético ou cirurgia faciais prévios e patologias neurológicas. Submeteram-se a 10 sessões de terapia, com exercícios estáticos e dinâmicos. Responderam questionário sobre modificações percebidas por elas ou referidas por terceiros. Suas fotos pré e pós tratamento foram analisadas por fonoaudiólogos especialistas em motricidade orofacial identificando presença ou ausência de modificações. Em escala análoga visual de 100 mm, marcaram seus graus de satisfação com aparência facial pré e pós tratamento. RESULTADOS: todas (100%) perceberam modificações faciais: diminuição das rugas dos olhos e dos lábios (100%) e diminuição do sulco nasolabial, lábios mais definidos, pele mais viçosa e brilhante e suavização das marcas de expressão (90...

Leptin and insulin in the seminal plasma of zebu bulls in peripuberty

Souza,Fernando Andrade; Martins,Jorge André Matias; Vale Filho,Vicente Ribeiro do; Andrade,Venício José de; Ferreira,Marcos Brandão Dias; Emerick,Lucas Luz; Pinto,Pablo Fabricio Bruno; Leite,Ticiano Guimarães
Fonte: Sociedade Brasileira de Zootecnia Publicador: Sociedade Brasileira de Zootecnia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/04/2012 Português
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In order to determine the profile of leptin and insulin values in seminal plasma and their correlations with sperm patterns during the peripuberty, a total of 16 crossbred Gyr dairy bulls were monitored from 60 days before to 60 days after puberty, separated into two groups, early and regular, according to the onset of puberty. Hormone values were determined by radioimmunoassay after gel filtration in fast performance liquid chromatography and semen patterns, according to the Brazilian College of Animal Reproduction. Insulin concentrations in seminal plasma did not differ between groups, nor between the ages, with the pooling of the data showing average of 35.15±16.93, 36.60±26.21, 26.82±09.81, and 43.56±31.71 32.24±16.71 U/mL for the pubertal period of -60, -30, 0, +30 and +60 days, respectively. These values were not correlated with sperm parameters, but differed in the group of animals with the highest percentage of major defects (31.08±18.58 U/mL) compared with those with lower percentage of these sperm defects (40.01±25.37 U/mL). Leptin was not correlated with sperm parameters and did not differ between groups or stage of sexual development, with concentrations of 23.10±10.43, 24.35±9.42, 22.41±9.90, 23.76±9.69 and 24.99±11.42 ng/mL for the periods of puberty aforementioned...

Thermodynamics of hydrogen cyanide and hydrogen fluoride binding to cytochrome c peroxidase and its Asn-82-->Asp mutant.

DeLauder, S F; Mauro, J M; Poulos, T L; Williams, J C; Schwarz, F P
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em 01/09/1994 Português
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The thermodynamics of binding of fluoride and cyanide to cytochrome c peroxidase (CCP) and its Asn-82-->Asp mutant (D82CCP) in phosphate and acetate buffer at an ionic strength of 0.15 mol.kg-1 from pH 5.0 to 7.1 were investigated by titration calorimetry at 289 and 297 K. The binding reactions are enthalpically driven. The fluoride-binding constants determined from the titration calorimetry results were in agreement with those determined from difference-spectroscopy measurements. For cyanide binding to CCP at 297.9 K, the binding constant decreased from 8.95 (+/- 0.83) x 10(5) M-1 at pH 7.0 to 4.04(+/- 0.23) x 10(5) M-1 at pH 5.0, and the binding enthalpy increased from -57.2 +/- 1.4 kJ.mol-1 at pH 7.0 to -48.6 +/- 1.8 kJ.mol-1 at pH 5.0. For fluoride binding to CCP, the binding constant increased from 8.41(+/- 0.54) x 10(3) M-1 at pH 7.0 to 3.11(+/- 0.09) x 10(5) M-1 at pH 5.0 and the binding enthalpy increased from -71.9 +/- 1.1 kJ.mol-1 at pH 7.0 to -67.0 +/- 1.9 kJ.mol-1 at pH 5.0. The binding enthalpies for D82CCP were about the same as those for CCP. However, the binding constants for cyanide and fluoride to D82CCP were respectively a factor of two less and at least an order of magnitude less than the corresponding binding constants of CCP. Decreased ligand-binding strength in the D82CCP mutant is thus entirely due to entropic effects.

2-(1H-1,2,3-Benzotriazol-1-yl)-1-(4-ethyl­benzo­yl)ethyl 2,4-dichloro­benzoate

Zeng, Wu-Lan
Fonte: International Union of Crystallography Publicador: International Union of Crystallography
Tipo: Artigo de Revista Científica
Publicado em 30/04/2008 Português
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In the title mol­ecule, C24H19Cl2N3, the dihedral angles between the benzotriazole group and the ethyl- and dichloro-substituted benzene rings are 16.53 (1) and 82.09 (1)°, respectively. The crystal structure is stabilized by weak inter­molecular C—H⋯O inter­actions.

2-Chloro-N′-(2-hydr­oxy-4-methoxy­benzyl­idene)benzohydrazide

Weng, Qianfeng; Zhao, Lei
Fonte: International Union of Crystallography Publicador: International Union of Crystallography
Tipo: Artigo de Revista Científica
Publicado em 19/03/2009 Português
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In the title compound, C15H13ClN2O3, the dihedral angle between the two benzene rings is 82.09 (10)° and an intra­molecular O—H⋯N hydrogen bond occurs. In the crystal structure, N—H⋯O hydrogen bonds link mol­ecules into chains propagating in [100].

{2,6-Bis[(diphenyl­phosphan­yl)­oxy]phen­yl-κ3 P,C 1,P′}iodidonickel(II)

Salah, Abderrahmen; Zargarian, Davit
Fonte: International Union of Crystallography Publicador: International Union of Crystallography
Tipo: Artigo de Revista Científica
Publicado em 12/03/2011 Português
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In the title complex, [Ni(C30H23O2P2)I], the divalent Ni atom is coordinated by two P atoms and one C atom from the 1,3-bis­[(diphenyl­phosphan­yl)­oxy]benzene ligand; the distorted square-planar geometry is completed by an iodide ligand. The largest distortions from ideal square-planar geometry are reflected in the P—Ni—P angle of 164.20 (2)° and the P—Ni—C angles of 82.09 (6) and 82.11 (6)°. The rather short Ni—C bond length [1.890 (2) Å] is anti­cipated in light of the much stronger trans influence of the aryl moiety compared to the iodide ligand. The P-bound phenyl rings adopt different orientations to minimize steric repulsion among themselves.

3-(4-Bromo­phenyl­sulfon­yl)-5-cyclo­pentyl-2-methyl-1-benzofuran

Choi, Hong Dae; Seo, Pil Ja; Lee, Uk
Fonte: International Union of Crystallography Publicador: International Union of Crystallography
Tipo: Artigo de Revista Científica
Publicado em 05/05/2012 Português
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In the title compound, C20H19BrO3S, the cyclo­pentyl ring adopts an envelope conformation. The 4-bromo­phenyl ring makes a dihedral angle of 82.09 (6)° with the mean plane [mean deviation = 0.026 (2) Å] of the benzofuran fragment. In the crystal, mol­ecules are linked by weak C—H⋯O hydrogen bonds and Br⋯O contacts [3.309 (2) Å].

(1S,8R,15S,19R)-17-Benzyl-17-aza­penta­cyclo­[6.6.5.02,7.09,14.015,19]nona­deca-2(7),3,5,9(14),10,12-hexa­ene chloro­form monosolvate

Bratko, Ielyzaveta; Ladeira, Sonia; Saffon, Nathalie; Teuma, Emmanuelle; Gómez, Montserrat
Fonte: International Union of Crystallography Publicador: International Union of Crystallography
Tipo: Artigo de Revista Científica
Publicado em 08/09/2012 Português
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In the title compound, C25H23N·CHCl3, the dihydro­anthracene unit is bent with a dihedral angle between the benzene rings of 57.82 (8)°. The N atom of the pyrrolidine heterocycle, which has an envelope conformation with the N atom as the flap, exhibits a pronounced pyramidalization [Σ(C—N—C) = 328.07°], indicating an accentuated N-donor character. In the crystal, this behaviour is evident by the C—H⋯N hydrogen bond involving a solvent mol­ecule and the N atom. The absolute configuration at the C-atom fused positions of the pyrrolidine group were crystallographically confirmed to be S and R.

6-Ferrocenoyl-7-(4-fluoro­phen­yl)spiro­[hexa­hydro­pyrrolo­[1,2-c][1,3]thia­zole-5,11′-indeno­[1,2-b]quinoxaline]

Suhitha, Sivasubramanian; Gunasekaran, Krishnaswamy; Gavaskar, Deivasigamani; Raghunathan, Raghavachary; Velmurugan, Devadasan
Fonte: International Union of Crystallography Publicador: International Union of Crystallography
Tipo: Artigo de Revista Científica
Publicado em 17/08/2013 Português
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In the title compound, [Fe(C5H5)(C32H23FN3OS)], both the thia­zolidine ring and the pyrrolidine ring adopt a twist conformation on the N—C(H) bridging bond. Their mean planes are inclined to one another by 10.05 (10)°, and they make dihedral angles of 82.09 (10) and 89.67 (11)°, respectively, with the cyclo­pentane ring. The F atom deviates by −0.0238 (2) Å from the benzene ring to which it is attached. In the crystal, mol­ecules are linked by a pair of C—H⋯O hydrogen bonds, forming inversion dimers.

Complete Genome Sequence of Streptococcus iniae YSFST01-82, Isolated from Olive Flounder in Jeju, South Korea

Rajoo, Sasikumar; Jeon, Wooyoung; Park, Kyungmoon; Yoo, Sungsik; Yoon, Injung; Lee, Hongweon; Ahn, Jungoh
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em 23/04/2015 Português
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Streptococcus iniae is associated with morbidity in commercial fish species, especially in olive flounders (Paralichthys olivaceus), and was recently identified as an emerging human pathogen. Here, we report the complete 2.09-Mb genome sequence of S. iniae strain YSFST01-82, isolated from an olive flounder with streptococcosis disease in Jeju, South Korea.

Patients’ preoperative perspectives concerning the decision to undergo total knee arthroplasty and comparison of their clinical assessments

Akbaba, Yıldız Analay; Yeldan, İpek; Özdinçler, Arzu Razak; Güney, Nejat
Fonte: The Society of Physical Therapy Science Publicador: The Society of Physical Therapy Science
Tipo: Artigo de Revista Científica
Português
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[Purpose] The aims of our study were, 1. to assess pain, limitation of movement ability, and functionality in osteoarthritis patients scheduled to undergo total knee arthroplasty, 2. to determine if pain (Group 1) or function loss (Group 2) has a greater influence on the decision of patients to have surgery, and 3. to compare results between Group 1 and Group 2. [Subject and Methods] Fifty-five osteoarthritis patients classified as grades 3 and 4 according to the Kellgren-Lawrence system of classification were evaluated for preoperative pain intensity with the Visual Analogue Scale, knee flexion/extension range of movement with a clinical goniometer, and function with the Western Ontario and McMaster Universities Osteoarthritis Index. Patients were examined to reveal their reasons regarding the decision to undergo total knee arthroplasty (pain or function loss). [Results] The Visual Analog Scale scores at rest and during activity were 5.62 and 7.42, the knee flexion range of movement and extension limitation were 93.17° and −7.04°, and the Western Ontario and McMaster Universities Osteoarthritis Index value was 82.09. Regarding the decision to undergo surgery, 47.3% (n=26) of the knees were in Group 1, and 52.7% were in Group 2; the two groups were not significantly different. There were also no significant differences between the groups in Visual Analog Scale score during activity...

Certidão de Julgamento n. 82/2005

Brasil. Superior Tribunal de Justiça (STJ)
Fonte: Superior Tribunal de Justiça do Brasil Publicador: Superior Tribunal de Justiça do Brasil
Formato: 6947 bytes; 19174 bytes; 3089 bytes; image/jpeg; image/jpeg; text/html
Português
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Ratificação de credenciamento de serviços médico-hospitalares. Processo n. 4170/2005. Julgado em 02/09/2005.

Ato n. 82, de 27 de maio de 2002

Brasil. Superior Tribunal de Justiça (STJ)
Fonte: Superior Tribunal de Justiça do Brasil Publicador: Superior Tribunal de Justiça do Brasil
Tipo: Legislação
Português
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Designa, Analista Judiciária, Área Judiciária, para exercer função comissionada de Assessor de Ministro, Código FC-09, junto ao Gabinete do Senhor Ministro Ari Pargendler, em vaga decorrente de dispensa de servidor.

Consumo e digestibilidade de aveia preta em caprinos de corte recebendo quatro níveis de suplemento

Luz, Paulo André Santos
Fonte: Dois Vizinhos Publicador: Dois Vizinhos
Tipo: Dissertação de Mestrado
Português
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The present study is to evaluate the intake and digestibility of oat IPR 61 (Avena strigosa), by meat goats receiving levels of concentrate supplementation.Was used eight goat males with nine months old and averaging 30 kg of body weight distributed by design completely randomized with four levels of concentrate intake: 0%, 0,5%%, 1%, 1,5%%, the BW. Trials were performed in IAPAR, Pato Branco, during the months from August to October in the years 2011 and 2012, a total of five periods valid observation.All treatments were given water and oats ad libtum fresh twice daily (8:30 am and 4pm) each treatment and their respective level of concentrate.Evaluation periods were fifteen days each ten days of adaptation and five data collection.There replacement ratio in the consumption of food by the use of concentrated 33%.The dry matter intake (DMI), organic matter, (OMI), digestible organic matter (DOMI), and organic matter roughage (OMRI) were influenced (P> 0.05) by concentrate level. The average consumption of these respective variables also suffered increasing influences of periods, and the DM content of forage (P> 0.05) and were higher for the period of five, when the DM content of the pasture was greater. Associative effect was found between levels of concentrate and parameters for organic matter digestibility: apparent (DOM)...

Clinical and epidemiological profile of patients with severe H1N1/09 pandemic influenza in Australia and New Zealand: an observational cohort study

Cheng, A.; Kotsimbos, T.; Reynolds, A.; Bowler, S.; Brown, S.; Hancox, R.; Holmes, M.; Irving, L.; Jenkins, C.; Thompson, P.; Simpson, G.; Waterer, G.; Wood-Baker, R.; Kelly, P.
Fonte: BMJ Group Publicador: BMJ Group
Tipo: Artigo de Revista Científica
Publicado em //2011 Português
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Background: Pandemic influenza H1N1/09 emerged in April 2009 and spread widely in Australia and New Zealand. Although an unprecedented number of cases required intensive care, comparative community-based studies with seasonal influenza strains have not shown any significant differences in clinical symptoms or severity. Methods: The authors performed active surveillance on confirmed influenza-related admissions and compared the clinical profile of patients with pandemic H1N1/09 influenza and patients with seasonal influenza at eight hospitals in Australia and one hospital in New Zealand. Results: During the 1 July and 30 November 2009, 560 patients with confirmed influenza were admitted, of which 478 had H1N1/09, and 82 had other seasonal strains. Patients with H1N1/09 influenza were younger, were more likely to have fever and were more likely to be pregnant but less likely to have chronic obstructive pulmonary disease and ischaemic heart disease than patients with seasonal strains. Other clinical features and comorbidities were reported in similar proportions. Admission to intensive care was required in 22% of patients with H1N1/09 influenza and 12% in patients with other strains. Hospital mortality was 5% in patients with H1N1 influenza. Conclusions: The clinical features of H1N1/09 influenza and seasonal strains were similar in hospitalised patients. A higher proportion of patients had comorbidities than had been reported in community-based studies. Although the overall mortality was similar...

Viajeros románticos a Oriente : Delacroix, Flaubert, Nerval / Nieves Soriano Nieto; director, Francisco Jarauta Marión.

Soriano Nieto, Nieves
Fonte: Murcia : Universidad de Murcia, Departamento de Filosofía, Publicador: Murcia : Universidad de Murcia, Departamento de Filosofía,
Tipo: Tese de Doutorado Formato: application/pdf
Português
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Texto en español, resumen en francés.; Tesis-Universidad de Murcia.; Consulte la tesis en: BCA. GENERAL. ARCHIVO UNIVERSITARIO. T.M. 3556.; Consulte la tesis en: BCA. GENERAL. DEPOSITO. DPT 20 367.; NEBRIJA. SALA SEGUNDA DERECHA. 82-9.0 SOR via-1.

Ecriture et mémoire : écrire après la Shoah

Abreu, Maria Manuela Almeida Alves de
Fonte: Universidade do Minho Publicador: Universidade do Minho
Tipo: Tese de Doutorado
Publicado em 29/01/2007 Português
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Tese de Doutoramento em Ciências da Literatura - Ramo do Conhecimento de Literatura Francesa; Le vaste domaine de la littérature des écrivains de la «deuxieme génération» de I'Holocauste est intimement marqué par la recherche des moyens d'expression de l'absence et du manque de tout ce qui a disparu. Robelt Bober, Raymond Federman, Patrick Modiano et Georges Perec engagent leur pratique littéraire dans un travail profond sur les modes d'écriture qui semblent être le seul moyen de parvenir à la résolution de la problématique de l' «indicible» qui obsede la plupart des auteurs de cette génération. Notre étude portera sur Quoi de neuf sur la guerre ? de Robert Bober, La voix dans le cabinet de débarras de Raymond Federman, Dora Bruder de Patrick Modiano et Récits d'Ellis Island de Georges Perec et Robert Bober. La Shoah et les traces qu'elle a laissées dans le monde d'aujourd'hui résonnent dans toutes ces reuvres tout en s'insérant dans un univers de la discrétion que nous considérons une «écriture en creux», c'est-à-dire une écriture marquée par la fragmentation, par l'obliquÍté et qui raconte sans raconter explicitement. Elle emploie des mécanismes et des structures qui révelent des points communs au sein de ce groupe d'auteurs...

Average and recommended half-life values for two neutrino double beta decay: upgrade-09

Barabash, A. S.
Fonte: Universidade Cornell Publicador: Universidade Cornell
Tipo: Artigo de Revista Científica
Publicado em 28/08/2009 Português
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All existing ``positive'' results on two neutrino double beta decay in different nuclei were analyzed. Using the procedure recommended by the Particle Data Group, weighted average values for half-lives of $^{48}$Ca, $^{76}$Ge, $^{82}$Se, $^{96}$Zr, $^{100}$Mo, $^{100}$Mo - $^{100}$Ru ($0^+_1$), $^{116}$Cd, $^{130}$Te, $^{150}$Nd, $^{150}$Nd - $^{150}$Sm ($0^+_1$) and $^{238}$U were obtained. Existing geochemical data were analyzed and recommended values for half-lives of $^{128}$Te, $^{130}$Te and $^{130}$Ba are proposed. We recommend the use of these results as presently the most precise and reliable values for half-lives.; Comment: 10 pages; talk at Matrix Elements for the Double-beta-decay Experiments (MEDEX'09), Prague, Czech Republic, June 15-19, 2009