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Anthocyanin pigments in strawberry

Lopes-da-Silva, M.F.; Escribano-Bailón, M.T.; Perez-Alonso, J.J.; Rivas-Gonzalo, J.C.; Santos-Buelga, Celestino
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Português
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The anthocyanin composition was analysed in strawberry fruits from five different cultivars (cv. Eris, Oso Grande, Carisma, Tudnew and Camarosa). Twenty-five defined anthocyanin pigments were detected, most of them containing Pelargonidin (Pg) as aglycone; some cyanidin (Cy) derivatives were also found. Glucose and rutinose were the usual substituting sugars, although arabinose and rhamnose were also tentatively identified; some minor anthocyanins showed acylation with aliphatic acids. A relevant aspect was the detection of anthocyanin-derived pigments, namely 5-carboxypyranopelargonidin-3-glucoside and four condensed pigments containing C–C linked anthocyanin (Pg) and flavanol (catechin and afzelechin) residues. Total anthocyanin content ranged between 200 and 600mg/kg, with Pg 3-gluc constituting 77–90% of the anthocyanins in the strawberry extracts followed by Pg 3-rut (6–11%) and Cy 3-gluc (3–10%). A notable variability was found among the anthocyanin concentrations in samples of a same variety and harvest, indicating a strongly influence of the degree of maturity, edaphic-climatic factors and post-harvest storage.

Flavanol-anthocyanin condensed pigments in plant extracts

González-Paramás, A.M.; Lopes-da-Silva, M.F.; Martin-Lopez, P.; Macz-Pop, G.; Gonzalez-Manzano, S.; Alcalde-Eon, C.; Perez-Alonso, J.J.; Escribano-Bailón, M.T.; Rivas-Gonzalo, J.C.; Santos-Buelga, Celestino
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Português
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37.144033%
Pigments resulting from the direct condensation of anthocyanins and flavanols are usually associated with reactions taking place during processing and storage of plant-derived foods and beverages and have been particularly studied in aged red wines. In this paper, small amounts of flavanol-anthocyanin condensed pigments are found in different plant extracts. Structures are suggested for 10 such condensed pigments detected in extracts of strawberry, runner beans, purple corn and grape skins, based on their MS" fragmentation patterns, following analyses by electrospray tandem mass spectrometry. All of them correspond to dimers containing a flavan-3-ol [either (epi)afzelechin, (epi)catechin or (epi)gallocatechin] as the upper unit carbon-carbon linked to a lower anthocyanin unit consisting of different delphinidin, cyanidin, pelargonidin, peonidin or malvidin derivatives. The detection of these pigments in plant extracts may suggest that they are natural pigments and not products exclusively formed during storage and ageing of processed foods and beverages, as was previously assumed.

Anthocyanin composition and related pigments in strawberry

Lopes-da-Silva, M.F.; Escribano-Bailón, M.T.; Martin-Lopez, P.; Rivas-Gonzalo, J.C.; Santos-Buelga, Celestino
Fonte: Sociedad Espanõla de Química Analítica Publicador: Sociedad Espanõla de Química Analítica
Tipo: Conferência ou Objeto de Conferência
Português
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37.247778%
The anthocyanin composition of the strawberry has been object of various studies, but it is still not fully characterized. It is well known the presence af pelarganidin 3 - glucoside (Pg 3 -gluc) as major anthocyanin, usually accompanied of smaller proportions of pelargonidin 3- rutinoside (Pg 3 -rut) and cyanidin 3-glucosid e (Cy. 3- gluc).

Hormonal modulation of photomorphogenesis-controlled anthocyanin accumulation in tomato (Solanum lycopersicum L. cv Micro-Tom) hypocotyls: Physiological and genetic studies

CARVALHO, Rogerio Falleiros; QUECINI, Vera; PERES, Lazaro Eustaquio Pereira
Fonte: ELSEVIER IRELAND LTD Publicador: ELSEVIER IRELAND LTD
Tipo: Artigo de Revista Científica
Português
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37.533857%
Hormones are likely to be important factors modulating the light-dependent anthocyanin accumulation. Here we analyzed anthocyanin contents in hypocotyls of near isogenic Micro-Tom (MT) tomato lines carrying hormone and phytochrome mutations, as single and double-mutant combinations. In order to recapitulate mutant phenotype, exogenous hormone applications were also performed Anthocyanin accumulation was promoted by exogenous abscisic acid (ABA) and inhibited by gibberellin (GA), in accordance to the reduced anthocyanin contents measured in ABA-deficient (notabills) and GA-constitutive response (procera) mutants. Exogenous cytokinin also enhanced anthocyanin levels in MT hypocotyls. Although auxin-insensitive chageotropica mutant exhibited higher anthocyanin contents, pharmacological approaches employing exogenous auxin and a transport inhibitor did not support a direct role of the hormone in anthocyanin accumulation Analysis of mutants exhibiting increased ethylene production (epwastic) or reduced sensitivity (Never ripe), together with pharmacological data obtained from plants treated with the hormone, indicated a limited role for ethylene in anthocyanin contents. Phytochrome-deficiency (aurea) and hormone double-mutant combinations exhibited phenotypes suggesting additive or synergistic interactions...

Estudo da interação entre fitocromo e hormônios vegetais no controle do desenvolvimento; Analysis of the interactions between phytochrome and plant hormones in plant development

Carvalho, Rogério Falleiros
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 24/01/2008 Português
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Muitas respostas moduladas pela luz durante o desenvolvimento das plantas também são reguladas por hormônios vegetais, levando à hipótese da interação entre ambos os fatores. Uma ferramenta valiosa para testar tal interação seria o uso de mutantes fotomorfogenéticos e hormonais, bem como duplos mutantes combinando ambos. Em tomateiro, embora sejam disponíveis mutantes com alterações na biossíntese de fotorreceptores e/ou na transdução do sinal da luz, bem como mutantes no metabolismo e/ou sensibilidade hormonal, esses estão presentes em cultivares diferentes, o que pode limitar seu uso de modo integrado e a construção de duplos mutantes. No presente trabalho, foram introgredidas em uma única cultivar de tomateiro, Micro-Tom (cv. MT), dezenove mutações afetando a biossíntese ou a resposta a fitocromo, bem como aos hormônios auxina (AUX), citocinina (CK), giberelina (GA), ácido abscísico (ABA), etileno (ET) e brassinoesteróides (BR). Tomando-se vantagem de tal coleção, duas respostas notadamente controladas tanto pela luz quanto por hormônios foram estudadas: alongamento e acúmulo de antocianinas em hipocótilos. Para tal, foram utilizadas as seguintes abordagens: i) tratamentos exógenos de diferentes classes hormonais em mutantes fotomorfogenéticos...

Determinação da composição de carotenoides e antocianinas de camu-camu (Myrciaria dubia).; Composition determination of carotenoids and anthocyanin in camu-camu (Myrciaria dubia).

Cinthia Fernanda Zanatta
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 08/10/2004 Português
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O camu-camu (Myrciaria dúbia) é uma pequena fruta arredondada, com coloração variando de avermelhada a roxo, que cresce em árvore nativa da região amazônica. Esta espécie tem despertado grande interesse devido ao seu elevado potencial funcional, principalmente por apresentar altos teores de ácido ascórbico, concentrações consideráveis de potássio, além da presença de carotenóides e antocianinas. Os carotenóides e as antocianinas não são apenas pigmentos naturais responsáveis pela coloração atrativa de frutas, flores e vegetais, mas são compostos que também desempenham diversas funções e ações benéficas ao ser humano. Como a composição completa dos pigmentos desta fruta ainda não foi estabelecida, o objetivo do presente trabalho foi separar, por cromatografia líquida de alta eficiência (CLAE), os carotenóides e antocianinas, caracterizar estes pigmentos e quantificá-los. Foram analisados frutos de camu-camu provenientes das cidades de Iguape e Mirandópolis, estado de São Paulo. Combinando a cromatografia em camada delgada e a CLAE em colunas de fase reversa C18 e C30, foram isolados 46 carotenóides; destes, 43 tiveram sua identidade confirmada e 3 não foram identificados. A all-trans-luteína foi o carotenóide principal encontrado nos frutos de camu-camu...

Possible influences of water loss and polyphenol oxidase activity on anthocyanin content and discoloration in fresh ripe strawberry (cv. Oso Grande) during storage at 1 degrees C

Nunes, M. Cecília N.; Brecht, Jeffrey K.; Morais, Alcina M. M. B.; Sargent, Steve A.
Fonte: Wiley Blackwell Publicador: Wiley Blackwell
Tipo: Artigo de Revista Científica
Publicado em //2005 Português
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Fresh‘Oso Grande’strawberries wrapped in polyvinyl chloride stretch film lost 0.7% of their initial weight during storage for 8 d at 1 °C, whereas unwrapped fruit lost 11%. Greater water loss was associated with darker and less red fruit, lower concentrations of anthocyanins and other soluble phenolics, and higher polyphenol oxidase (PPO) activity. Anthocyanin degradation and oxidation of soluble phenolic compounds, caused possibly by increased PPO activity as a result of water loss, contributed to the development of strawberry surface browning during storage. Proper handling to reduce water loss during postharvest operations should be used to maintain acceptable color of strawberries during shipping and retailing.

Ultraviolet-B-Responsive Anthocyanin Production in a Rice Cultivar Is Associated with a Specific Phase of Phenylalanine Ammonia Lyase Biosynthesis.

Reddy, V. S.; Goud, K. V.; Sharma, R.; Reddy, A. R.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /08/1994 Português
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Seedlings of 17 rice (Oryza sativa L.) cultivars were classified on the basis of anthocyanin pigmentation into three groups: an acyanic group with 9 cultivars, a moderately cyanic group with 5 cultivars, and a cyanic group with 3 cultivars. Seedlings of the cyanic group were deep purple in color, possessing copious amounts of anthocyanin in shoots. Sunlight (SL)-mediated anthocyanin and phenylalanine ammonia lyase (PAL) induction in a cyanic cultivar, purple puttu, was compared with an acyanic cultivar, black puttu. A brief exposure of dark-grown purple puttu seedlings to SL induced anthocyanin formation during a subsequent dark period with a peak at 24 h. The magnitude of SL-mediated anthocyanin induction is age dependent, the 4-d-old seedlings being the most responsive to SL. The anthocyanin induction in purple puttu seedlings is mediated exclusively by the ultraviolet-B (UV-B) component of SL. The SL-triggered anthocyanin induction was reduced by about 30% by a terminal far-red light pulse and was restored by a red light pulse, indicating the role of phytochrome in modulation of anthocyanin level. The SL-mediated induction of PAL showed two peaks, one at 4 h and the other at 12 h. Whereas the first PAL peak (4 h) was induced by phytochrome and was seen in both cultivars...

Activation of Anthocyanin Biosynthesis in Gerbera hybrida (Asteraceae) Suggests Conserved Protein-Protein and Protein-Promoter Interactions between the Anciently Diverged Monocots and Eudicots1

Elomaa, Paula; Uimari, Anne; Mehto, Merja; Albert, Victor A.; Laitinen, Roosa A.E.; Teeri, Teemu H.
Fonte: The American Society for Plant Biologists Publicador: The American Society for Plant Biologists
Tipo: Artigo de Revista Científica
Publicado em /12/2003 Português
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We have identified an R2R3-type MYB factor, GMYB10, from Gerbera hybrida (Asteraceae) that shares high sequence homology to and is phylogenetically grouped together with the previously characterized regulators of anthocyanin pigmentation in petunia (Petunia hybrida) and Arabidopsis. GMYB10 is able to induce anthocyanin pigmentation in transgenic tobacco (Nicotiana tabacum), especially in vegetative parts and anthers. In G. hybrida, GMYB10 is involved in activation of anthocyanin biosynthesis in leaves, floral stems, and flowers. In flowers, its expression is restricted to petal epidermal cell layers in correlation with the anthocyanin accumulation pattern. We have shown, using yeast (Saccharomyces cerevisiae) two-hybrid assay, that GMYB10 interacts with the previously isolated bHLH factor GMYC1. Particle bombardment analysis was used to show that GMYB10 is required for activation of a late anthocyanin biosynthetic gene promoter, PGDFR2. cis-Analysis of the target PGDFR2 revealed a sequence element with a key role in activation by GMYB10/GMYC1. This element shares high homology with the anthocyanin regulatory elements characterized in maize (Zea mays) anthocyanin promoters, suggesting that the regulatory mechanisms involved in activation of anthocyanin biosynthesis have been conserved for over 125 million years not only at the level of transcriptional regulators but also at the level of the biosynthetic gene promoters.

Mode of coaction between blue/UV light and light absorbed by phytochrome in light-mediated anthocyanin formation in the milo (Sorghum vulgare Pers.) seedling

Oelmüller, Ralf; Mohr, Hans
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /09/1985 Português
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27.513088%
Anthocyanin formation in milo (Sorghum vulgare Pers.) seedlings (coleoptile, mesocotyl, taproot) occurs only in white light and blue/UV light (BL/UV), while red light (RL) and far-RL are totally ineffective. However, after a BL/UV pretreatment, the participation of phytochrome can be demonstrated. With a short-wavelength light source [peak emission in longwave UV (UV-A)], the mode of coaction between BL/UV and light absorbed by phytochrome (RL) was studied with the following principal results. (i) As soon as the seedling becomes competent to respond to UV-A (with regard to anthocyanin formation), the involvement of phytochrome can be detected. (ii) A 5-min pulse of UV-A has a strong effect on the anthocyanin synthesis in the milo mesocotyl. This effect is fully reversible if a long-wavelength far-RL pulse (RG9 light) is given immediately after the UV-A light pulse. (iii) When seedlings treated with 5 min of UV-A and 5 min of RG9 light are kept in darkness for 3 hr and then transferred to RL, anthocyanin appears. (iv) In continuous UV-A treatment, anthocyanin accumulation starts after a lag phase of 3.5 hr (25°C). A RL pretreatment prior to the onset of UV-A treatment strongly increases anthocyanin accumulation in UV-A, though the lag phase is not affected. Moreover...

Ethylene Suppression of Sugar-Induced Anthocyanin Pigmentation in Arabidopsis1[C][W][OA]

Jeong, Seok-Won; Das, Prasanta Kumar; Jeoung, Sae Chae; Song, Ji-Young; Lee, Hyun Kyoung; Kim, Yeon-Ki; Kim, Woo Jung; Park, Yong Il; Yoo, Sang-Dong; Choi, Sang-Bong; Choi, Giltsu; Park, Youn-Il
Fonte: American Society of Plant Biologists Publicador: American Society of Plant Biologists
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
27.48861%
Anthocyanin accumulation is regulated negatively by ethylene signaling and positively by sugar and light signaling. However, the antagonistic interactions underlying these signalings remain to be elucidated fully. We show that ethylene inhibits anthocyanin accumulation induced by sucrose (Suc) and light by suppressing the expression of transcription factors that positively regulate anthocyanin biosynthesis, including GLABRA3, TRANSPARENT TESTA8, and PRODUCTION OF ANTHOCYANIN PIGMENT1, while stimulating the concomitant expression of the negative R3-MYB regulator MYBL2. Genetic analyses show that the ethylene-mediated suppression of anthocyanin accumulation is dependent upon ethylene signaling components responsible for the triple response. Furthermore, these positive and negative signaling pathways appear to be under photosynthetic control. Suc and light induction of anthocyanin accumulation was almost fully inhibited in wild-type Arabidopsis (Arabidopsis thaliana) ecotype Columbia and ethylene (ethylene response1 [etr1-1]) and light (long hypocotyl1 [hy1], cryptochrome1/2, and hy5) signaling mutants treated with the photosynthetic electron transport inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea. The transcript level of the sugar transporter gene SUC1 was enhanced in ecotype Columbia treated with the ethylene-binding inhibitor silver and in etr1-1...

Different Localization Patterns of Anthocyanin Species in the Pericarp of Black Rice Revealed by Imaging Mass Spectrometry

Yoshimura, Yukihiro; Zaima, Nobuhiro; Moriyama, Tatsuya; Kawamura, Yukio
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 17/02/2012 Português
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27.48861%
Black rice (Oryza sativa L. Japonica) contains high levels of anthocyanins in the pericarp and is considered an effective health-promoting food. Several studies have identified the molecular species of anthocyanins in black rice, but information about the localization of each anthocyanin species is limited because methodologies for investigating the localization such as determining specific antibodies to anthocyanin, have not yet been developed Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) is a suitable tool for investigating the localization of metabolites. In this study, we identified 7 species of anthocyanin monoglycosides and 2 species of anthocyanin diglycosides in crude extracts from black rice by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) analysis. We also analyzed black rice sections by MALDI-IMS and found 2 additional species of anthocyanin pentosides and revealed different localization patterns of anthocyanin species composed of different sugar moieties. Anthocyanin species composed of a pentose moiety (cyanidin-3-O-pentoside and petunidin-3-O-pentoside) were localized in the entire pericarp, whereas anthocyanin species composed of a hexose moiety (cyanidin-3-O-hexoside and peonidin-3-O-hexoside) were focally localized in the dorsal pericarp. These results indicate that anthocyanin species composed of different sugar moieties exhibit different localization patterns in the pericarp of black rice. This is the first detailed investigation into the localization of molecular species of anthocyanins by MALDI-IMS.

Occurrence of anthocyanin pigments in berries of the white cultivar Muscat Gordo Blanco (Vitis vinifera L.)

Gholami, Mansour; Coombe, B. G.
Fonte: Australian Society of Viticulture & Oenology Publicador: Australian Society of Viticulture & Oenology
Tipo: Artigo de Revista Científica
Publicado em //1995 Português
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37.144033%
Rose coloration of skin was observed to sometimes develop late during ripening on the normally white grape berries of cv. Muscat Gordo Blanco. The nature of the pigment was investigated by HPLC analysis of skin extracts of single berries. The predominant anthocyanin was identified as cyanidin-3-glucoside with minor amounts of delphinidin- and peonidin-3-glucosides. This composition resembles the skin composition of coloured, small-seeded muscat cultivars which it also resembles by the monoterpene composition of the juice. The pigments occurred only in berries with levels of total soluble solids in excess of 24°Brix in the juice and such berries tended to have smaller fresh weight. Berry pigmentation occurred on vines with various root systems. The specific conditions under which pigment developed in Muscat Gordo berries may offer a useful tool in the study of anthocyanin biosynthesis.

Purification, molecular cloning, and characterization of glutathione S-transferases (GSTs) from pigmented Vitis vinifera L. cell suspension cultures as putative anthocyanin transport proteins

Conn, S.; Curtin, C.; Beizer, A.; Franco, C.; Zhang, W.
Fonte: Oxford Univ Press Publicador: Oxford Univ Press
Tipo: Artigo de Revista Científica
Publicado em //2008 Português
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37.322705%
The ligandin activity of specific glutathione S-transferases (GSTs) is necessary for the transport of anthocyanins from the cytosol to the plant vacuole. Five GSTs were purified from Vitis vinifera L. cv. Gamay Fréaux cell suspension cultures by glutathione affinity chromatography. These proteins underwent Edman sequencing and mass spectrometry fingerprinting, with the resultant fragments aligned with predicted GSTs within public databases. The corresponding coding sequences were cloned, with heterologous expression in Escherichia coli used to confirm GST activity. Transcriptional profiling of these candidate GST genes and key anthocyanin biosynthetic pathway genes (PAL, CHS, DFR, and UFGT) in cell suspensions and grape berries against anthocyanin accumulation demonstrated strong positive correlation with two sequences, VvGST1 and VvGST4, respectively. The ability of VvGST1 and VvGST4 to transport anthocyanins was confirmed in the heterologous maize bronze-2 complementation model, providing further evidence for their function as anthocyanin transport proteins in grape cells. Furthermore, the differential induction of VvGST1 and VvGST4 in suspension cells and grape berries suggests functional differences between these two proteins. Further investigation of these candidate ligandins may identify a mechanism for manipulating anthocyanin accumulation in planta and in vitro suspension cells.; Simon Conn...

Microwave-assisted extraction of anthocyanin from Chinese bayberry and its effects on anthocyanin stability

DUAN,Wenkai; JIN,Shiping; ZHAO,Guofu; SUN,Peilong
Fonte: Sociedade Brasileira de Ciência e Tecnologia de Alimentos Publicador: Sociedade Brasileira de Ciência e Tecnologia de Alimentos
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/09/2015 Português
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37.379355%
AbstractAnthocyanins are present in high concentrations in Chinese bayberry, Myrica rubra Sieb. & Zucc. Herein, a microwave-assisted extraction was used to extract the anthocyanins from Chinese bayberry. The HPLC chromatogram of the extracts showed that the anthocyanin components were slightly hydrolysed during the extraction process. Further experiments confirmed that microwave irradiation slightly hydrolysed cyanidin-3-O-glucoside to cyanidin, but did not significantly influence the antioxidant activity of the extracts. Optimized extraction conditions for total anthocyanin content were a solid-to-liquid ratio, extraction temperature, and extraction time of 1:50, 80 °C, and 15 min, respectively. Under these conditions, the anthocyanin content was 2.95 ± 0.08 mg·g−1, and the antioxidant activity yield was 279.96 ± 0.1 μmol.·g−1 Trolox equivalent on a dry weight basis. These results indicated that microwave-assisted extraction was a highly efficient extraction method with reduced processing time. However, under some extraction conditions it could damage the anthocyanins. These results provide an important guide for the application of microwave extraction.

LcMYB1 Is a Key Determinant of Differential Anthocyanin Accumulation among Genotypes, Tissues, Developmental Phases and ABA and Light Stimuli in Litchi chinensis

Lai, Biao; Li, Xiao-Jing; Hu, Bing; Qin, Yong-Hua; Huang, Xu-Ming; Wang, Hui-Cong; Hu, Gui-Bing
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 21/01/2014 Português
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27.567197%
The red coloration of litchi fruit depends on the accumulation of anthocyanins. The anthocyanins level in litchi fruit varies widely among cultivars, developmental stages and environmental stimuli. Previous studies on various plant species demonstrate that anthocyanin biosynthesis is controlled at the transcriptional level. Here, we describe a litchi R2R3-MYB transcription factor gene, LcMYB1, which demonstrates a similar sequence as other known anthocyanin regulators. The transcription levels of the LcMYB1 and anthocyanin biosynthetic genes were investigated in samples with different anthocyanin levels. The expression of LcMYB1 was strongly associated with tissue anthocyanin content. LcMYB1 transcripts were only detected in anthocyanin-accumulating tissues and were positively correlated with anthocyanin accumulation in the pericarps of 12 genotypes. ABA and sunlight exposure promoted, whereas CPPU and bagging inhibited the expression of LcMYB1 and anthocyanin accumulation in the pericarp. Cis-elements associated with light responsiveness and abscisic acid responsiveness were identified in the promoter region of LcMYB1. Among the 6 structural genes tested, only LcUFGT was highly correlated with LcMYB1. These results suggest that LcMYB1 controls anthocyanin biosynthesis in litchi and LcUFGT might be the structural gene that is targeted and regulated by LcMYB1. Furthermore...

Varietal differences among the anthocyanin profiles of 50 red table grape cultivars studied by high performance liquid chromatography

Pomar, F.; Novo, M.; Masa Vázquez, Antón
Fonte: Elsevier Publicador: Elsevier
Tipo: Artículo Formato: 37888 bytes; application/msword
Português
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In order to develop a method that allows to distinguish between grape cultivars, the anthocyanin profiles of 50 accessions from the “Misión Biológica de Galicia” germplasm collection were studied by high performance liquid chromatography (HPLC). Nineteen anthocyanins were totally or partly identified and significant quantitative differences between the studied anthocyanin markers were found. With this method all 50 cultivars examinated could be easily distinguished from each other. In addition, the HPLC fingerprints and the relative-area anthocyanins plot for every cultivar has been elaborated and stored in a database. To test the validity of this method, several unknown samples have been analysed comparing their anthocyanin profile with the fingerprint database, and we may conclude that this has been proved to be of great value for grape cultivar recognition.; This study was financially supported by the project XUGA-40301B97 from the Galician government in Spain and partially by the project RF99-110 (INIA) from the Spanish government.; Peer reviewed

EFEITO DE ANTOCIANINA E PRÓPOLIS EM DIABETES INDUZIDA EM COELHOS; EFFECTS OF ANTHOCYANIN AND PROPOLIS IN DIABETIC RABBITS

Oliveira, Tânia Toledo de; Nagem, Tanus Jorge; Pinto, Aloísio Silva; Message, Dejair; Tinoco, Adelson Luiz Araújo; Magalhães, Neuza Maria; Silva, José Francisco; Huertas, Alfredo Alcides Goicochea; Pinto, José Geraldo Pinto Geraldo; Pezerico, Gracie
Fonte: Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto Publicador: Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; Formato: application/pdf
Publicado em 30/12/2002 Português
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37.247778%
A síndrome clínica da diabetes é caracterizada pela redução da capacidade de metabolização de carboidratos e gorduras, resultando em aumento dos níveis séricos de glicose (hiperglicemia) e lipídios (hiperlipidemia), acarretando em degeneração vascular prematura. O objetivo do presente trabalho foi testar os efeitos da antocianina e do própolis ministrados nas doses de 20mg e 150mg, respectivamente, em coelhos diabéticos para a verificação dos efeitos sobre os níveis de glicose e triacilglicerol. Os grupos experimentais de coelhos estudados foram: Grupo 1- grupo controle que somente recebeu ração; Grupo 2- grupo diabético que recebeu ração mais aloxano; Grupo 3- grupo diabético que recebeu ração, aloxano e 1 cápsula de antocianina (20mg) diariamente; Grupo 4- grupo diabético que recebeu ração, aloxano e 1 cápsula de própolis (150mg) diariamente. Os Grupos 3 e 4 foram submetidos a vinte e oito dias de tratamento, com dosagem semanal dos níveis sanguíneos de glicose e de triacilglicerol. De acordo com os resultados obtidos, pode-se observar que, durante todo o período experimental, a antocianina foi mais eficaz, em induzir a queda dos níveis de glicose, com reduções de 10,78% aos 7 dias, 10,54% aos 14 dias...

Anthocyanin regulatory gene expression in transgenic white clover can result in an altered pattern of pigmentation.

de Majnik, J; Weinman, Jeremy; Djordjevic, Michael; Rolfe, Barry; Tanner, Gregory J; Joseph, Roslyn G; Larkin, Philip J
Fonte: CSLI Publications Publicador: CSLI Publications
Tipo: Artigo de Revista Científica
Português
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37.48861%
This study presents the first evidence of heterologous anthocyanin regulatory genes altering anthocyanin expression in stably transformed leguminous plants. Two families of anthocyanin regulatory genes, myc (delila, B-Peru) and myb (myb. Ph2, Cl), are involved in the activation of the phenylpropanoid pathway. White clover (Trifolium repens cv. Haifa) plants were transformed with dicotyledonous and monocotyledonous myb or myc genes. Some of these transformed plants exhibited enhanced anthocyanin accumulation in a range of tissues. One plant, transformed with the B-Peru gene driven by the Cauliflower Mosaic Virus 35S promoter, displayed a unique pattern of anthocyanin accumulation in the leaf. The accumulation of anthocyanin in this plant was closely associated with the crescent of leaves, which is normally white. The red pigmentation declined in intensity in the oldest leaf stage. The B-Peru message was detected in all leaf stages of this white clover plant. This anthocyanin pattern was shown to be heritable.

Effect of convective drying on total anthocyanin content, antioxidant activity and cell morphometric parameters of strawberry parenchymal tissue (Fragaria x ananassa Dutch)

Morales-Delgado,D.Y.; Téllez-Medina,D.I.; Rivero-Ramírez,N. L.; Arellano-Cárdenas,S.; López-Cortez,S.; Hernández-Sánchez,H.; Gutiérrez-López,G.; Cornejo-Mazón,M.
Fonte: UAM, Unidad Iztapalapa, División de Ciencias Básicas e Ingeniería Publicador: UAM, Unidad Iztapalapa, División de Ciencias Básicas e Ingeniería
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2014 Português
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The effect of convective drying of strawberries (Fragaria x ananassa Dutch) on the contents of anthocyanins (AcyTot) and antioxidant capacity (TEAC), as equivalents of Trolox, was evaluated as well as its relation to cell morphological changes in the mesocarp by means of determining the variations in Area (A), Perimeter (P), Feret Diameter (Fe) and Fractal Dimension of parenchymal tissue cells. A decrease in anthocyanin content caused by drying at 60, 70 80 and 90 °C and 1 m/s airflow was observed. ACyTot and TEAC showed correlation (r = 0.784). The decrease in ACyTot and TEAC in samples dehydrated at 60 °C, was associated to the decrease in values of A, P, Fe and FD found in samples dried at this temperature whereas no changes in A, P and Fe were found in samples dried at 70-90 °C in relation to those observed at 60 °C. The cell contour resulted smoother after high-temperature drying, as indicated by the decrease in FD. The antioxidant activity related to changes in ACyTot and TEAC took place at progressively higher levels in samples that changed morphology in the same proportion among each other.