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Electrospray Ionization Mass Spectrometry Fingerprinting of Extracts of the Leaves of Arrabidaea chica

Schiozer, Adriana L.; Cabral, Elaine C.; de Godoy, Luiz A. F.; Chaves, Francisco C. M.; Poppi, Ronei J.; Riveros, Jose M.; Eberlin, Marcos N.; Barata, Lauro E. S.
Fonte: SOC BRASILEIRA QUIMICA; SAO PAULO Publicador: SOC BRASILEIRA QUIMICA; SAO PAULO
Tipo: Artigo de Revista Científica
Português
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Arrabidaea chica (crajiru) is an important Amazonian plant. Its extracts are used as red pigments, antimicrobial agents and astringents. Three different varieties of this species are cultivated in the Amazon region. In this work, direct infusions of A. chica extracts from these three varieties were analyzed via electrospray ionization mass spectrometry (ESI(+)-MS) fingerprinting. Derived data from the spectra were classified by using a multivariate method (PLS-DA, partial least squares-discriminant analysis). The direct method that is herein presented relies on extraction of dry, powdered leaves with acidified methanol/water solution with no further sample preparation. The resulting supernatants were analyzed by direct infusion ESI(+)-MS, which provides characteristic fingerprints of the sample composition. 3-Deoxyanthocyanidins are important substances in A. chica, their ions were used as markers in the PLS-DA data treatment. PLS-DA was able to differentiate the three varieties. ESI(+)-MS fingerprinting works as a simple and fast method to differentiate varieties of A. chica.; Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES); Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)

Determinação da atividade antimicrobiana in vitro de extratos, frações e compostos isolados de Arrabidaea brachypoda

Rozatto, Mariana Rodrigues
Fonte: Universidade Estadual Paulista (UNESP) Publicador: Universidade Estadual Paulista (UNESP)
Tipo: Dissertação de Mestrado Formato: 100 f. : il., grafs.
Português
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Pós-graduação em Ciências Farmacêuticas - FCFAR; As plantas são uma fonte importante de produtos naturais biologicamente ativos, muitos dos quais derivam diversos fármacos. A grande diversidade encontrada no Brasil justifica o crescimento significativo na utilização de produtos de origem vegetal como potenciais fitoterápicos. O gênero Arrabidaea pertence à família Bignoniaceae, a qual é encontrada principalmente em regiões tropicais. No Brasil, são encontrados 56 gêneros e cerca de 338 espécies. As plantas desta família são aplicadas como adstringentes, antitérmicas e no tratamento de reumatismos, diarreias, câncer e infecções microbianas; suas propriedades são atribuídas aos componentes químicos dentre estes as lignanas, flavonoides, triterpenos, xantonas entre outros. Arrabidaea brachypoda é popularmente conhecida como “cervejinha do campo”, “cipó-una” ou “tintureiro”. Com o objetivo de contribuir com a avaliação do potencial farmacobiológico do Bioma Cerrado e proposição de novos fitoterápicos para uso popular, este estudo visou determinar a atividade antimicrobiana de extratos hidroalcoólicos, frações e substâncias isoladas da planta A. brachypoda...

Destanização do caqui (Diospyrus kaki L.) "Rama Forte"

Victor Ricardo de Souza Muñoz
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em /12/2002 Português
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As cultivares adstringentes de caqui (Diospyros kaki L.) apresentam, como principal característica, altos teores de taninos solúveis, requerendo portanto, um processo artificial de destanização. Dentre as cultivares adstringentes, destaca-se a ‘Rama Forte’, que representa 60 % do caqui comercializado no Estado de São Paulo. Entretanto, além dos preços baixos durante o pico de safra, o caqui enfrenta ainda alguns problemas no processo de destanização. A falta de conhecimento e tecnologia disponível aos produtores faz com que sejam aplicadas técnicas rudimentares que, eliminam a adstringência, mas promovem um amolecimento do fruto, impedindo sua conservação por períodos prolongados. A bibliografia cita várias técnicas de destanização, como etanol, etileno e CO2, entretanto, não há referencias para as cultivares produzidas no Brasil. Frente a este contexto, elaborou-se este trabalho com o objetivo de definir as melhores condições (tempo de exposição e concentração) para estes métodos de destanização e avaliar a melhor forma de conservação do fruto por períodos prolongados. Para a seleção das melhores condições de destanização e conservação dos frutos foram avaliados o índice de adstringência e outros índices de qualidade durante os processos de destanização e conservação. Na determinação das melhores condições de destanização para cada método...

Electrospray Ionization Mass Spectrometry Fingerprinting of Extracts of the Leaves of Arrabidaea chica

Schiozer, Adriana L.; Cabral, Elaine C.; de Godoy, Luiz A. F.; Chaves, Francisco C. M.; Poppi, Ronei J.; Riveros, Jose M.; Eberlin, Marcos N.; Barata, Lauro E. S.
Fonte: Soc Brasileira Quimica; Sao Paulo Publicador: Soc Brasileira Quimica; Sao Paulo
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
16.958953%
Arrabidaea chica (crajiru) is an important Amazonian plant. Its extracts are used as red pigments, antimicrobial agents and astringents. Three different varieties of this species are cultivated in the Amazon region. In this work, direct infusions of A. chica extracts from these three varieties were analyzed via electrospray ionization mass spectrometry (ESI(+)-MS) fingerprinting. Derived data from the spectra were classified by using a multivariate method (PLS-DA, partial least squares-discriminant analysis). The direct method that is herein presented relies on extraction of dry, powdered leaves with acidified methanol/water solution with no further sample preparation. The resulting supernatants were analyzed by direct infusion ESI(+)-MS, which provides characteristic fingerprints of the sample composition. 3-Deoxyanthocyanidins are important substances in A. chica, their ions were used as markers in the PLS-DA data treatment. PLS-DA was able to differentiate the three varieties. ESI(+)-MS fingerprinting works as a simple and fast method to differentiate varieties of A. chica.

Electrospray ionization mass spectrometry fingerprinting of extracts of the leaves of Arrabidaea chica

Schiozer,Adriana L.; Cabral,Elaine C.; Godoy,Luiz A. F. de; Chaves,Francisco C. M.; Poppi,Ronei J.; Riveros,José M.; Eberlin,Marcos N.; Barata,Lauro E. S.
Fonte: Sociedade Brasileira de Química Publicador: Sociedade Brasileira de Química
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/03/2012 Português
Relevância na Pesquisa
16.958953%
Arrabidaea chica (crajiru) is an important Amazonian plant. Its extracts are used as red pigments, antimicrobial agents and astringents. Three different varieties of this species are cultivated in the Amazon region. In this work, direct infusions of A. chica extracts from these three varieties were analyzed via electrospray ionization mass spectrometry (ESI(+)-MS) fingerprinting. Derived data from the spectra were classified by using a multivariate method (PLS-DA, partial least squares-discriminant analysis). The direct method that is herein presented relies on extraction of dry, powdered leaves with acidified methanol/water solution with no further sample preparation. The resulting supernatants were analyzed by direct infusion ESI(+)-MS, which provides characteristic fingerprints of the sample composition. 3-Deoxyanthocyanidins are important substances in A. chica, their ions were used as markers in the PLS-DA data treatment. PLS-DA was able to differentiate the three varieties. ESI(+)-MS fingerprinting works as a simple and fast method to differentiate varieties of A. chica.

Practical Uses of Botanicals in Skin Care

Stallings, Alison F.; Lupo, Mary P.
Fonte: Matrix Medical Communications Publicador: Matrix Medical Communications
Tipo: Artigo de Revista Científica
Publicado em /01/2009 Português
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Cosmeceuticals are the fastest growing sector of the cosmetic industry, and the future of antiaging cosmeceuticals in particular is very promising. Botanical extracts that support the health, texture, and integrity of the skin, hair, and nails are widely used in cosmetic formulations. They form the largest category of cosmeceutical additives found in the marketplace today due to the rising consumer interest and demand for natural products. Various plant extracts that formed the basis of medical treatments in ancient civilizations and many traditional cultures are still used today in cleansers, moisturizers, astringents, and many other skin care products. New botanical skin care treatments are emerging, presenting dermatologists and their patients the challenge of understanding the science behind these cosmeceuticals. Thus, dermatologists must have a working knowledge of these botanicals and keep up with how they evolve to provide optimal medical care and answer patient questions. The most popular botanicals commonly incorporated into skin care protocols are discussed.

Composi??o qu?mica atividades antioxidante e alelop?tica da caroba do campo (Jacaranda caroba (Vell.) A. DC. ? Bignoniaceae)

Bento, Cec?lia de Souza Oliveira; Miranda, Roqueline Rodrigues Silva de
Fonte: UFVJM Publicador: UFVJM
Tipo: Dissertação de Mestrado
Português
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ABSTRACT Brazil is a country with significant biodiversity and is one of its Cerrado biomes that presents great diversity of native plant species, many used in folk medicine and with little or no scientific study. Among these species, there is the Jacaranda carob (Vell.) A. DC. (Bignoniaceae), popularly known as "carob", "carobinha" and "carob field." In folk medicine against impigens is used as astringents and wound healing, as a diuretic for the treatment of infections, syphilis and ulcers. Some phytochemical studies revealed the presence of phenolic compounds and terpenoids in kind and not biological studies have been published about the same so far. Thus, this study had as objective to evaluate the chemical composition of plant extracts and antioxidant potential survey and allelopathic species. The samples of plant material were carried out in natural habitat, in the Campus JK UFVJM in Diamantina-MG. In phytochemical studies, samples were evaluated and nonpolar components analysis proposed by GC / MS, IR and NMR, indicating the likely presence of aliphatic and unsaturated, straight chain and branched ketones, organic acids, esterified or not (such as decanoic acid) and phenolic compounds (such as hydroquinone). Through studies of antioxidant activity was observed that the polar extracts of leaves and flowers of J. caroba present phenolic compounds with antioxidant potential. Was observed allelopathic activity of alcoholic and hydroalcoholic extracts of flowers and leaves J. carob seeds on germination and the growth or development of plants.; O Brasil ? um dos pa?ses com expressiva biodiversidade e o Cerrado ? um dos seus biomas que apresenta grande diversidade de esp?cies vegetais nativas...

Potencial inflamat??rio de duas t??cnicas para afastamento gengival utilizadas durante procedimentos prot??ticos; Inflammatory potential of two techniques used for gingival retraction during restorative procedures

SARMENTO, Hugo Ramalho
Fonte: Universidade Federal de Pelotas; Odontologia; Programa de P??s-Gradua????o em Odontologia; UFPel; BR Publicador: Universidade Federal de Pelotas; Odontologia; Programa de P??s-Gradua????o em Odontologia; UFPel; BR
Tipo: Dissertação Formato: application/pdf
Português
Relevância na Pesquisa
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This study was composed by two phases that evaluated two gingival displacement (GD) techniques, conventional and cordless: 1- a in vitro study which assess cytoxicity/genotoxicity, and pH of AlCl3-based agents; and 2- a randomized clinical which evaluated the efficacy of these techniques. For in vitro phase, eluates were obtained by dilution of AlCl3-based into culture medium for 1h, 4h, and 24h. pH values were measured by digital pHmeter, cytotoxicity by MTT test and genotoxicity by Micronuclei test. A crossover, double-blind, randomized clinical trial was designed to evaluate clinical, immunologic and individual factors before and after GD by two techniques, conventional (gingival cord + astringent gel) and cordless (astringent-based paste). These factors were evaluated on incisors prepared for metal-ceramic dental crowns before, 1 day post-GD and 10 day post-GD. Interleukin 1β, Interleukin 6 and Tumor Necrosis Factor α concentrations were evaluated on gingival crevicular fluid collected before (control) and 1 day post-GD. In vitro data were analyzed by two-way ANOVA and Tukey test. Clinical data were analyzed by One-way Repeated Measures ANOVA and Tukey test (immunologic factor), Friedman test (periodontal parameters)...

Astringency and other oral sensations : biological sources of individual variation and association with food and beverage behaviour

Bajec, Marth R.
Fonte: Brock University Publicador: Brock University
Tipo: Electronic Thesis or Dissertation
Português
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Orosensory perception strongly influences liking and consumption of foods and beverages. This thesis examines the influence of biological sources of individual variation on the perception of prototypical orosensory stimuli, food liking, self-reported alcohol liking and consumption, and indices of health. Two orosensory indices were examined: propylthiouracil (PROP) responsiveness, a genetically-mediated index of individual variation associated with enhanced responsiveness to orosensory stimuli often expressed as PROP taster status (PTS); and thermal taster status (TTS), a recently reported index of orosensory responsiveness. Taster status in PTS and/or TTS confers greater responsiveness to most orosensory stimuli. Gender, age, ethnicity, and fungiform papillae (FP) density were not associated with orosensory responsiveness to tastants, an astringent, and a flavour. Unlike PROP responsiveness, FP density was not associated with TTS. Both PROP responsiveness and TTS were associated with increased responsiveness to orosensory stimuli, including temperature and astringency. For PROP, this association did not hold when stimuli were presented at cold or warm temperatures...