Por meio de cineventriculografia esquerda em posição oblíqua anterior direita (OAD) a 30º, foram avaliados o tamanho, forma e movimentação regional do ventrículo esquerdo (VE) de um grupo de 15 pacientes chagásicos cronicos sintomáticos (Grupo Chagásico) e de um grupo de 11 indivíduos submetidos a estudo hemodinâmico e angiocaroiografia por suspeita de doença cardíaca, mas cujos resultados foram normais (Grupo Controle). Os parâmetros analisados foram: volume diastólico final (VDF), volume sistólico final (VSF), fração de ejeção (FE), excentricidade, sentido das curvaturas dos contornos das silhuetas, percentagem de encurtamento segmentar ao longo da sistole, a cada 31 ms, velocidade média (normalizada) de encurtamento segmentar (VME), sequência de encurtamento segmentar, volume e forma das regiões apicais acineticas e discinéticas. Foram também correlacionados os sintomas dos pacientes, eletrocaroiograma (ECG), RX de área cardiaca e pressoes do VE e da Aorta. Para o estudo da movimentação regional foi idealizado um novo método, cujos pontos principais foram: 1) suposição de que o ápex anatômico permanece praticamente fixo durante a sístole; 2) divisão das silhuetas por eixos longitudinais ligando a base (região mitro-aórtica) e a extremidade apical angiografica...

O objetivo neste trabalho foi avaliar o efeito do período de descanso (3, 5, 7 e 9 horas) dos suínos no frigorífico (PDF) e da localização dos suínos na carroceria do caminhão (PBO), quando transportados, no inverno ou verão, sobre alguns parâmetros fisiológicos avaliados em 64 fêmeas, com peso médio de 130kg para abate, durante o manejo pré-abate. Para a análise estatística, foram considerados, no modelo de análise da variância, os efeitos de bloco, PDF, PBO e da interação (bloco x PDF), entre outros. O PDF influenciou, significativamente, as concentrações de lactato no sangue e cortisol na saliva. Suínos que descansaram 5 e 7 horas apresentaram maior concentração de lactato em relação aos animais que descansaram 3 e 9 horas. No transporte, a freqüência cardíaca foi muito maior em relação aos demais locais avaliados. Concluiu-se que o incremento do PDF não promove mudanças na freqüência cardíaca, nas concentrações de glicose e CPK no sangue e cortisol na saliva, mas interfere na concentração de lactato no sangue dos suínos.

Peptide deformylase (PDF) is an essential bacterial metalloenzyme which deformylates the N-formylmethionine of newly synthesized polypeptides and as such represents a novel target for antibacterial chemotherapy. To identify novel PDF inhibitors, we screened a metalloenzyme inhibitor library and identified an N-formyl-hydroxylamine derivative, BB-3497, and a related natural hydroxamic acid antibiotic, actinonin, as potent and selective inhibitors of PDF. To elucidate the interactions that contribute to the binding affinity of these inhibitors, we determined the crystal structures of BB-3497 and actinonin bound to Escherichia coli PDF at resolutions of 2.1 and 1.75 Å, respectively. In both complexes, the active-site metal atom was pentacoordinated by the side chains of Cys 90, His 132, and His 136 and the two oxygen atoms of N-formyl-hydroxylamine or hydroxamate. BB-3497 had activity against gram-positive bacteria, including methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus faecalis, and activity against some gram-negative bacteria. Time-kill analysis showed that the mode of action of BB-3497 was primarily bacteriostatic. The mechanism of resistance was via mutations within the formyltransferase gene...

Intraperitoneal antibiotics are used to treat Pseudomonas aeruginosa peritonitis, a serious complication of continuous ambulatory peritoneal dialysis. However, P. aeruginosa killing is often inefficient despite low MBCs. Broth dilution MIC/MBC and time kill curves of tobramycin, amikacin, netilmicin, azlocillin, piperacillin, ceftazidime, cefsulodin, and ciprofloxacin were determined in peritoneal dialysis fluid (PDF), buffered PDF, fluid recovered from patients on continuous ambulatory peritoneal dialysis (RPF), and cation-supplemented Mueller-Hinton broth. MBCs of all antibiotics were 8 to 16 times greater in PDF and RPF than in Mueller-Hinton broth or buffered PDF. Use of the time kill curve technique and Mueller-Hinton broth showed that aminoglycosides killed greater than or equal to 99.9% of P. aeruginosa at 1 h, ciprofloxacin killed greater than or equal to 99.9% at 2 h, and beta-lactams killed greater than or equal to 99.9% at 6 h. In contrast, killing was not demonstrated in PDF by any drug at 6 h and by aminoglycosides only at 24 h. Bactericidal activity was optimal in RPF for ciprofloxacin at 1 h and for aminoglycosides at 2 h; bactericidal activity was not demonstrated in RPF with any beta-lactam (no kill by penicillins; less than 99% kill by cephalosporins). Slow bacterial growth...

The authors present a segment-based convolution method to account for the interplay effect between intrafraction organ motion and the multileaf collimator position for each particular segment in intensity modulated radiation therapy (IMRT) delivered in a step-and-shoot manner. In this method, the static dose distribution attributed to each segment is convolved with the probability density function (PDF) of motion during delivery of the segment, whereas in the conventional convolution method (“average-based convolution”), the static dose distribution is convolved with the PDF averaged over an entire fraction, an entire treatment course, or even an entire patient population. In the case of IMRT delivered in a step-and-shoot manner, the average-based convolution method assumes that in each segment the target volume experiences the same motion pattern (PDF) as that of population. In the segment-based convolution method, the dose during each segment is calculated by convolving the static dose with the motion PDF specific to that segment, allowing both intrafraction motion and the interplay effect to be accounted for in the dose calculation. Intrafraction prostate motion data from a population of 35 patients tracked using the Calypso system (Calypso Medical Technologies...

Tip60 is a histone acetyltransferase (HAT) enzyme that epigenetically regulates genes enriched for neuronal functions through interaction with the amyloid precursor protein (APP) intracellular domain. However, whether Tip60-mediated epigenetic dysregulation affects specific neuronal processes in vivo and contributes to neurodegeneration remains unclear. Here, we show that Tip60 HAT activity mediates axonal growth of the Drosophila pacemaker cells, termed “small ventrolateral neurons” (sLNvs), and their production of the neuropeptide pigment-dispersing factor (PDF) that functions to stabilize Drosophila sleep–wake cycles. Using genetic approaches, we show that loss of Tip60 HAT activity in the presence of the Alzheimer’s disease-associated APP affects PDF expression and causes retraction of the sLNv synaptic arbor required for presynaptic release of PDF. Functional consequence of these effects is evidenced by disruption of the sleep–wake cycle in these flies. Notably, overexpression of Tip60 in conjunction with APP rescues these sleep–wake disturbances by inducing overelaboration of the sLNv synaptic terminals and increasing PDF levels, supporting a neuroprotective role for dTip60 in sLNv growth and function under APP-induced neurodegenerative conditions. Our findings reveal a novel mechanism for Tip60 mediated sleep–wake regulation via control of axonal growth and PDF levels within the sLNv-encompassing neural network and provide insight into epigenetic-based regulation of sleep disturbances observed in neurodegenerative diseases like Alzheimer’s disease.

The probability density function (PDF), and its corresponding cumulative density function (CDF), provide direct statistical insight into the characterization of a random process or field. Typically displayed as a histogram, one can infer probabilities of the occurrence of particular events. When examining a field over some two-dimensional domain in which at each point a PDF of the function values is available, it is challenging to assess the global (stochastic) features present within the field. In this paper, we present a visualization system that allows the user to examine two-dimensional data sets in which PDF (or CDF) information is available at any position within the domain. The tool provides a contour display showing the normed difference between the PDFs and an ansatz PDF selected by the user and, furthermore, allows the user to interactively examine the PDF at any particular position. Canonical examples of the tool are provided to help guide the reader into the mapping of stochastic information to visual cues along with a description of the use of the tool for examining data generated from an uncertainty quantification exercise accomplished within the field of electrophysiology.

Histamine and its two receptors, histamine-gated chloride channel subunit 1 (HisCl1) and ora transientless (Ort), are known to control photoreception and temperature sensing in Drosophila. However, histamine signaling in the context of neural circuitry for sleep-wake behaviors has not yet been examined in detail. Here, we obtained mutant flies with compromised or enhanced histamine signaling and tested their baseline sleep. Hypomorphic mutations in histidine decarboxylase (HDC), an enzyme catalyzing the conversion from histidine to histamine, caused an increase in sleep duration. Interestingly, hisCl1 mutants but not ort mutants showed long-sleep phenotypes similar to those in hdc mutants. Increased sleep duration in hisCl1 mutants was rescued by overexpressing hisCl1 in circadian pacemaker neurons expressing a neuropeptide pigment dispersing factor (PDF). Consistently, RNA interference (RNAi)-mediated depletion of hisCl1 in PDF neurons was sufficient to mimic hisCl1 mutant phenotypes, suggesting that PDF neurons are crucial for sleep regulation by the histamine-HisCl1 signaling. Finally, either hisCl1 mutation or genetic ablation of PDF neurons dampened wake-promoting effects of elevated histamine signaling via direct histamine administration. Taken together...

Nonlinear Processes in Geophysics, European Geosciences Union/American Geophysical Union, 12, 1-10.; Probabilistic aspects of regional ocean model predictability is analyzed using the probability density function (PDF) of the irreversible predictability time (IPT) (called t -PDF) computed from an unconstrained ensemble of stochastic perturbations in initial conditions, winds, and open boundary conditions. Two-attractors (a chaotic attractor and a small-amplitude stable limit cycle) are found in the winddriven circulation. Relationship between attractor’s residence time and IPT determines the -PDF for the short (up to several weeks) and intermediate (up to two months) predictions. The t-PDF is usually non-Gaussian but not multimodal for red-noise perturbations in initial conditions and perturbations in the wind and open boundary conditions. Bifurcation of t -PDF occurs as the tolerance level varies. Generally, extremely successful predictions (corresponding to the t-PDF’s tail toward large IPT domain) are not outliers and share the same statistics as a whole ensemble of predictions.

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Processo FAPESP: 2009/16206-1; Processo FAPESP: 2010/02045-3; Processo FAPESP: 2011/14058-5; Processo FAPESP: 2011/14094-1; Processo FAPESP: 2013/20387-7; We propose a nature-inspired approach to estimate the probability density function (pdf) used for data clustering based on the optimum-path forest algorithm (OPFC). OPFC interprets a dataset as a graph, whose nodes are the samples and each sample is connected to its k-nearest neighbors in a given feature space (a k-nn graph). The nodes of the graph are weighted by their pdf values and the pdf is computed based on the distances between the samples and their k-nearest neighbors. Once the k-nn graph is defined, OPFC finds one sample (root) at each maximum of the pdf and propagates one optimum-path tree (cluster) from each root to the remaining samples of its dome. Clustering effectiveness will depend on the pdf estimation, and the proposed approach efficiently computes the best value of k for a given application. We validate our approach in the context of intrusion detection in computer networks. First...

Column-density maps of molecular clouds are one of the most important observables in the context of molecular cloud- and star-formation (SF) studies. With the Herschel satellite it is now possible to determine the column density from dust emission. We use observations and simulations to demonstrate how LOS contamination affects the column density probability distribution function (PDF). We apply a first-order approximation (removing a constant level) to the molecular clouds of Auriga, Maddalena, Carina and NGC3603. In perfect agreement with the simulations, we find that the PDFs become broader, the peak shifts to lower column densities, and the power-law tail of the PDF flattens after correction. All PDFs have a lognormal part for low column densities with a peak at Av~2, a deviation point (DP) from the lognormal at Av(DP)~4-5, and a power-law tail for higher column densities. Assuming a density distribution rho~r^-alpha, the slopes of the power-law tails correspond to alpha(PDF)=1.8, 1.75, and 2.5 for Auriga, Carina, and NGC3603 (alpha~1.5-2 is consistent gravitational collapse). We find that low-mass and high-mass SF clouds display differences in the overall column density structure. Massive clouds assemble more gas in smaller cloud volumes than low-mass SF ones. However...

Nanocrystalline n-AlO(OH) (Disperal P2(R) of Sasol) was investigated by means of the atomic pair distribution function (PDF). The PDF is derived from powder diffraction data, an ideally resolved PDF is obtained from a synchrotron source which provides a large maximal scattering vector length Qmax > 300 nm-1. Here, however, we were able to reveal atomic structure details of the about 4 nm particles from in-house diffraction data (Qmax = 130 nm-1): PDF least squares model refinements show that n-AlO(OH) is of the layered Boehmite structure type (oC16, Cmcm). But the structure is uniformly distorted in domains of ca. 2 nm size within the nano particles. The hydrogen bonds between the layers are widened up significantly by +13 pm, accounting for the higher reactivity when compared to microcrystalline Boehmite. Our results from only one "nanocrystallographic" experiment are consistent with a trend which was found via the Rietveld technique on a series of AlO(OH) of different crystallite size (Bokhimi et al., 2001). In addition, the PDF contains information on structural distortion as a function of (coherence) domain size within the nano particles.

We present the first unbiased determination of parton distribution functions (PDFs) with electroweak corrections. The aim of this thesis is to provide an exhaustive description of the theoretical framework and the technical implementation which leads to the determination of a set of PDFs which includes the photon PDF and quantum electrodynamics (QED) contributions to parton evolution. First, we introduce and motivate the need of including electroweak corrections to PDFs, providing phenomenological examples and presenting an overview of the current state of the art in PDF fits. The theoretical implications of such corrections are then described through the implementation of the combined QCD+QED evolution in APFEL, a public code for the solution of the PDF evolution developed particularly for this thesis. We proceed by presenting the new structure of the Neural-Network PDF (NNPDF) methodology used for the extraction of this set of PDFs with QED corrections. We then provide a first determination of the full set of PDFs based on deep-inelastic scattering data and LHC data for $W$ and $Z/\gamma^*$ Drell-Yan production, using leading-order QED and NLO or NNLO QCD: the so-called NNPDF2.3QED set of PDFs. We perform a preliminary investigation of the phenomenological implications of NNPDF2.3QED set...

We study the ellipticity probability distribution function (PDF) of voids in redshift space with galaxies as tracers of the shapes of voids. We find that the redshift space distortion on the shape of voids statistically increases the ellipticities of voids, and leaves a prominent feature on the ellipticity PDF as a substantial reduction in the probability of having voids with small ellipticity. The location of this characteristic cutoff of the ellipticity PDF is an explicit function of the logarithmic growth rate, and it can be used as a probe of cosmology once the radial density profile of voids is better understood. However, the biggest limiting factor for the use of ellipticity PDF as a probe of cosmology lies in the Poisson noise from a small number of galaxies to define the shape of a given void. This Poisson noise creates a significant contamination of the resulting ellipticity PDF so that the shape of the original PDF is almost washed-out. Nevertheless, there is a way to overcome the Poisson noise via the Alcock Paczynski test on the shape of stacked voids. In redshift space, since the void is elongated toward the line of sight, the stacked void has non-zero ellipticity, which can be a tell-tale of the logarithmic growth rate. Although some useful information of void ellipticity will be lost by stacking...

The probability distribution function (PDF) of the mass surface density is an essential characteristic of the structure of molecular clouds or the interstellar medium in general. Observations of the PDF of molecular clouds indicate a composition of a broad distribution around the maximum and a decreasing tail at high mass surface densities. The first component is attributed to the random distribution of gas which is modeled using a log-normal function while the second component is attributed to condensed structures modeled using a simple power-law. The aim of this paper is to provide an analytical model of the PDF of condensed structures which can be used by observers to extract information about the condensations. The condensed structures are considered to be either spheres or cylinders with a truncated radial density profile at cloud radius r_cl. The assumed profile is of the form rho(r)=rho_c/(1+(r/r_0)^2)^{n/2} for arbitrary power n where rho_c and r_0 are the central density and the inner radius, respectively. An implicit function is obtained which either truncates (sphere) or has a pole (cylinder) at maximal mass surface density. The PDF of spherical condensations and the asymptotic PDF of cylinders in the limit of infinite overdensity rho_c/rho(r_cl) flattens for steeper density profiles and has a power law asymptote at low and high mass surface densities and a well defined maximum. The power index of the asymptote Sigma^(-gamma) of the logarithmic PDF (Sigma x P(Sigma)) in the limit of high mass surface densities is given by gamma = (n+1)/(n-1)-1 (spheres) or by gamma=n/(n-1)-1 (cylinders in the limit of infinite overdensity).; Comment: 12 pages...

The Fortran LHAPDF library has been a long-term workhorse in particle physics, providing standardised access to parton density functions for experimental and phenomenological purposes alike, following on from the venerable PDFLIB package. During Run 1 of the LHC, however, several fundamental limitations in LHAPDF's design have became deeply problematic, restricting the usability of the library for important physics-study procedures and providing dangerous avenues by which to silently obtain incorrect results. In this paper we present the LHAPDF 6 library, a ground-up re-engineering of the PDFLIB/LHAPDF paradigm for PDF access which removes all limits on use of concurrent PDF sets, massively reduces static memory requirements, offers improved CPU performance, and fixes fundamental bugs in multi-set access to PDF metadata. The new design, restricted for now to interpolated PDFs, uses centralised numerical routines and a powerful cascading metadata system to decouple software releases from provision of new PDF data and allow completely general parton content. More than 200 PDF sets have been migrated from LHAPDF 5 to the new universal data format, via a stringent quality control procedure. LHAPDF 6 is supported by many Monte Carlo generators and other physics programs...

Detecting the dark matter annihilation signal from Galactic substructure, or subhalos, is an important challenge for high-energy gamma-ray experiments. In this paper we discuss detection prospects by combining two different aspects of the gamma-ray signal: the angular distribution and the photon counts probability distribution function (PDF). The true PDF from subhalos has been shown recently (by Lee et al.) to deviate from Poisson; we extend this analysis and derive the signal PDF from a detailed Lambda-CDM-based model for the properties of subhalos. We combine our PDF with a model for Galactic and extra-Galactic diffuse gamma-ray emission to obtain an estimator and projected error on dark matter particle properties (mass and annihilation cross section) using the Fermi Gamma-Ray Space Telescope. We compare the estimator obtained from the true PDF to that obtained from the simpler Poisson analysis. We find that, although both estimators are unbaised in the presence of backgrounds, the error on dark matter properties derived from the true PDF is ~50% smaller than when utilizing the Poisson-based analysis.

We present a near-infrared extinction map of a large region ($\sim$870 deg$^2$) covering the isolated Corona Australis complex of molecular clouds. We reach a 1-$\sigma$ error of 0.02 mag in the K-band extinction with a resolution of 3 arcmin over the entire map. We find that the Corona Australis cloud is about three times as large as revealed by previous CO and dust emission surveys. The cloud consists of a 45 pc long complex of filamentary structure from the well known star forming Western-end (the head, $N \geq10^{23}$ cm$^{-2}$) to the diffuse Eastern-end the tail, ($N \leq10^{21}$ cm$^{-2}$). Remarkably, about two thirds of the complex both in size and mass lie beneath A$_V\sim1$ mag. We find that the PDF of the cloud cannot be described by a single log-normal function. Similar to prior studies, we found a significant excess at high column densities, but a log-normal + power-law tail fit does not work well at low column densities. We show that at low column densities near the peak of the observed PDF, both the amplitude and shape of the PDF are dominated by noise in the extinction measurements making it impractical to derive the intrinsic cloud PDF below A$_K <$ 0.15 mag. Above A$_K \sim 0.15$ mag, essentially the molecular component of the cloud...

This article describes the results of an action research carried out to elaborate an educational material on Intravenous Therapy displayed on the Internet, and its evaluation, considering its usefulness as a subsidiary tool in nursing learning-teaching process, not only in nursing schools settings but also in services, in ongoing education programs. The study was developed through data base search and image collection with a digital camera, in the nursing laboratory and at the Western Parana University Hospital (Hospital Universitário do Oeste do Paraná). The material was organized in modules, and the results are published in the following electronic address: http:// www.unioeste.br/projetos/terapiaintravenosa/pdf/1.pdf. After displaying it on the Internet, it was evaluated by students and professors who considered its use valid as a supporting material for the learning process of Intravenous Therapy.; Investigación y acción realizadas para elaboración de material educativo acerca de la Terapia Intravenosa (TIV), tornándolo disponible en la Internet, y la evaluación del mismo, considerando el objetivo de utilizarlo como herramienta para subsidiar el proceso de enseñanza y aprendizaje de enfermería, no sólo en el ámbito de las escuelas...

TODESCHINI, Giacomo. Commerciare nell’Occidente medievale: Il Sacro quotidiano. Disponível em: http://dipeco.economia.unimib.it/pdf/iniziative/todescihini%20wp.pdf. Acessado em agosto de 2012. Tradução portuguesa de Selvino J. Assmann; TODESCHINI, Giacomo. Commerciare nell’Occidente medievale: Il Sacro quotidiano. Disponível em: http://dipeco.economia.unimib.it/pdf/iniziative/todescihini%20wp.pdf. Acessado em agosto de 2012. Tradução portuguesa de Selvino J. Assmann; http://dx.doi.org/10.5007/1807-1384.2013v10n2p409TODESCHINI, Giacomo. Commerciare nell’Occidente medievale: Il Sacro quotidiano. Disponível em: http://dipeco.economia.unimib.it/pdf/iniziative/todescihini%20wp.pdf. Acessado em agosto de 2012. Tradução portuguesa de Selvino J. AssmannORIGENS MEDIEVAIS DA RACIONALIDADE ECONÔMICA MODERNAApresentamos a tradução de um historiador italiano, Giacomo Todeschini, que gentilmente nos cedeu o direito de apresentar um texto representativo de uma obra que traz um tema recorrente: o da origem medieval e, digamos, católica da racionalidade econômica moderna e capitalista.  Esta posição assumida pelo autor insere-se num debate importante em curso, em que desponta a interpretação feita por Max Weber no início do século XX...